Comparative study on HIV rapid detection of saliva and blood in MSM of Shenzhen

Abstract

Objective To compare the results of HIV rapid detection reagent in saliva and blood in MSM in Shenzhen, and we clarify the role of saliva rapid detection in HIV screening in this population. Methods The exudate in saliva and blood samples of the same subjects in 393 MSM in Shenzhen were collected, and the HIV immunoblotting kit was used as the reference reagent to confirm the consistency of salivary antibody detection kit (colloidal gold method) and human immunodeficiency virus (HIV) type 1+2 antibody detection kit (colloidal selenium method). Results Totally 393 MSM were recruited. The true positive, false positive, true negative, false negative cases for saliva test reagents were 120, 0, 272, 1, respectively. Those for blood test reagents were 121, 3, 269, 0, respectively. The detection results of the HIV western blot kit were taken as the gold standard to compare and analyze the detection results of saliva and blood detection reagent. The sensitivity, specificity, false-positive rate, false-negative rate and accuracy of exudate in saliva test reagents were 99.2%, 100.0%, 0.0%, 0.8% and 99.8%, respectively. Those of blood test reagents were 100.0%, 98.9%, 1.10%, 0.0% and 99.2%, respectively. Kappa test analysis showed that the results of rapid saliva and blood test were consistent with HIV diagnosis, and the Kappa values were 0.994 and 0.982, respectively. McNemar paired chi-square test was used to analyze the results of saliva and blood rapid test, and the difference was not statistically significant ( P =0.125). Conclusion Saliva and blood screening results have a good consistency, exudate in saliva detection is noninvasive and fast, this method is worth promoting for HIV self-screening in high risk population. 摘要：目的 分析深圳市男男性行为者(MSM)中唾液和血液HIV快速检测试剂结果, 探讨唾液快速检测法在MSM 人群HIV筛査的作用。 方法 同时采集深圳市393名MSM人群的唾液和血液样本 , 使用HIV免疫印迹试剂盒作为参 考试剂, 比较唾液抗体检测试剂盒(胶体金法)和人类免疫缺陷病毒(HIV) 1+2型抗体检测试剂盒(胶体砸法)两种快速 检测试剂结果的符合性。 结果 在393份MSM有效样本中, 唾液检测试剂的真阳性为120例, 假阳性为0例, 真阴性为 272例, 假阴性为1例。血液快速法真阳性为121例, 假阳性为3例, 真阴性为269例, 假阴性为0例。以HIV免疫印迹试 剂盒检测结果为金标准 , 唾液检测试剂的灵敏度为99.2%, 特异度为100.0%, 假阳性率为0.0%, 假阴性率为0.8%, 准确 度为99.8%。血液检测试剂的灵敏度为100.0%,特异度为98.9%, 假阳性率为1.1%, 假阴性率为0.0%, 准确度为 99.2%。Kappa检验分析显示唾液、血液快速检测法与HIV诊断结果存在一致性, Kappa值分别为0.994和0.982; McNemar配对卡方检验分析唾液和血液快速法的检测结果发现二者的差异无统计学意义( P =0.125)。 结论 唾液与血液的 筛査结果具有较好一致性 , 基于唾液检测安全无创、快速, 该方法值得在髙危人群HIV抗体自我筛査中推广使用