Atcharaporn Srijudanu, O. Piasai, W. Leesutthiphonchai, Mattana Wanitch, C. Chaisiri, N. Khewkhom
{"title":"Biocontrol of Colletotrichum falcatum Causing Red Rot Disease of Sugarcane Using Non-toxigenic Aspergillus sp.","authors":"Atcharaporn Srijudanu, O. Piasai, W. Leesutthiphonchai, Mattana Wanitch, C. Chaisiri, N. Khewkhom","doi":"10.12982/cmjs.2023.032","DOIUrl":null,"url":null,"abstract":"Red rot disease caused by Colletotrichum falcatum is a major production threat to several sugarcane varieties in Thailand. Biological control is an ecologically friendly alternative to manage the disease using microbial antagonism and associated metabolic products. In this study, four Aspergillus non-toxigenic strains (A37, A374, A400, and A561) were characterized using a genetic approach and evaluated for their antagonistic potential to suppress the growth of C. falcatum. The strain A561 had the maximum number of gene deletions, with 12 afl atoxin-producing genes being lost. In addition, the non-toxigenic characteristic of A561 was confi rmed using afl atoxin B1 (AFB1) enzyme-linked immunosorbent assay. A561 reduced the concentration of AFB1 by 98.57% (1.63 μg/kg) relative to the toxigenic control. The antagonistic activity of A561 was evaluated against C. falcatum using the competition plate method on potato dextrose agar (PDA) in vitro. The results revealed that A561 inhibited the mycelial growth of C. falcatum by 34.39%. In addition, 10% (v/v) culture fi ltrate of A561 on PDA produced mycelial inhibition of 53.21%. Non-toxigenic A561 had antifungal potential, suppressing the growth of C. falcatum.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2023.032","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Red rot disease caused by Colletotrichum falcatum is a major production threat to several sugarcane varieties in Thailand. Biological control is an ecologically friendly alternative to manage the disease using microbial antagonism and associated metabolic products. In this study, four Aspergillus non-toxigenic strains (A37, A374, A400, and A561) were characterized using a genetic approach and evaluated for their antagonistic potential to suppress the growth of C. falcatum. The strain A561 had the maximum number of gene deletions, with 12 afl atoxin-producing genes being lost. In addition, the non-toxigenic characteristic of A561 was confi rmed using afl atoxin B1 (AFB1) enzyme-linked immunosorbent assay. A561 reduced the concentration of AFB1 by 98.57% (1.63 μg/kg) relative to the toxigenic control. The antagonistic activity of A561 was evaluated against C. falcatum using the competition plate method on potato dextrose agar (PDA) in vitro. The results revealed that A561 inhibited the mycelial growth of C. falcatum by 34.39%. In addition, 10% (v/v) culture fi ltrate of A561 on PDA produced mycelial inhibition of 53.21%. Non-toxigenic A561 had antifungal potential, suppressing the growth of C. falcatum.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.