Isolation and Characterization of Phosphate Solubilizing Bacteria from Calcareous Soil

S. Gaikwad, P. Bhosale, P. Ukey, K. B. Landage
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Abstract

The present investigation was conducted with aim to isolate and characterize phosphate solubilizing bacteria from calcareous soil. Four efficient PSB isolates were obtained from fourteen soil samples collected from different locations. Based on morphological and biochemical characterization, highly phosphate solubilizing isolates were identified as Pseudomonas fluorescence, Bacillus subtilis, Bacillus thuringiensis and Pseudomonas aeruginosa respectively. The amount of Pi released from TCP by the isolates at 10 DAI ranged from 23.2 to 30.5 % and zone of solubilization recorded between 6 -13 mm. Among them PSB isolate 1 (Pseudomonas fluorescence) recorded highest Pi released from TCP broth i:e (30.5 %) and zone of solubilization recorded (13 mm) than the other isolates tested.
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钙质土壤中增磷菌的分离与特性研究
本研究旨在从钙质土壤中分离和鉴定增磷菌。从不同地点的14份土壤样品中分离出4株高效PSB菌株。通过形态学和生化鉴定,鉴定出高溶磷菌株分别为荧光假单胞菌、枯草芽孢杆菌、苏云金芽孢杆菌和铜绿假单胞菌。在10 DAI时,分离物从TCP中释放的Pi量为23.2% ~ 30.5%,增溶区为6 ~ 13 mm。其中PSB菌株1(荧光假单胞菌)在TCP肉汤中释放的Pi最高(30.5%),溶出区(13 mm)高于其他菌株。
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