Screening a novel high effective dioscorea zingiberensis degradation strain

Yanjun Lu, Jifeng Guo
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Abstract

12 strains of cellulolytic microorganism were isolated from the AB reactor of dioscorea zingiberensis wastewater treatment. A bacterial strain was obtained through the screening experiments of decomposition of dioscorea zingiberensis, which had high degradability for dioscorea zingiberensis and was named D-1. The gene sequence obtained from the DNA extraction and polymerase chain reaction (PCR) amplification was compared with NCBI Genbank of U.S.A., and the strain was preliminary identified to be Clostridium cellulosolvens. The optimum growth conditions were pH 5.0, 30°C, 100 r/min.
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一株高效薯蓣降解菌株的筛选
从盾叶薯蓣废水处理的AB反应器中分离出12株纤维素降解微生物。通过对盾叶薯蓣的分解筛选实验,获得了一株对盾叶薯蓣具有较高降解率的菌株,命名为D-1。通过DNA提取和聚合酶链反应(PCR)扩增获得的基因序列与美国NCBI Genbank进行比对,初步鉴定该菌株为Clostridium cellulosolvens。最佳生长条件为pH 5.0, 30℃,100 r/min。
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