{"title":"Analysis of cell metabolic products and fermentation gases by gas chromatography","authors":"P. Unger, Z. Vozňáková, J. Páca","doi":"10.1002/JBT.2570270122","DOIUrl":null,"url":null,"abstract":"A method for the estimation of ethanol and volatile fatty acids by gas chromatography by direct treatment of cultivation broth is described. The packing material was Hallcomid M-18 with 1% H3PO4 on Chromosorb W AW. The separation was made at 132°C with nitrogen as a carrier gas; for detection a flame ionisation detector was used. The analysis of oxygen and carbon dioxide of the inlet and outlet gas from a fermentor was also studied. For the separation of the gases the combination of molecular seive 5A and Chromosorb 102 was used. The analysis was performed at 35°C with helium as a carrier gas and a thermal conductivity detector was used. With the experimental conditions found in this study the methods described gave reproducible and accurate results for the analytical control of cell cultivation processes.","PeriodicalId":15255,"journal":{"name":"Journal of biochemical toxicology","volume":"27 1","pages":"150-154"},"PeriodicalIF":0.0000,"publicationDate":"2007-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biochemical toxicology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/JBT.2570270122","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
Abstract
A method for the estimation of ethanol and volatile fatty acids by gas chromatography by direct treatment of cultivation broth is described. The packing material was Hallcomid M-18 with 1% H3PO4 on Chromosorb W AW. The separation was made at 132°C with nitrogen as a carrier gas; for detection a flame ionisation detector was used. The analysis of oxygen and carbon dioxide of the inlet and outlet gas from a fermentor was also studied. For the separation of the gases the combination of molecular seive 5A and Chromosorb 102 was used. The analysis was performed at 35°C with helium as a carrier gas and a thermal conductivity detector was used. With the experimental conditions found in this study the methods described gave reproducible and accurate results for the analytical control of cell cultivation processes.
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