Study of Thioredoxin Isolated from Human Blood Serum

Abstract

The research included the separation of thioredoxin (Trx) from human blood serum using different biochemical techniques. One proteinous peak had been isolated by gel filtration using sephadex )G-50) that produced by ammonium sulphate precipitation(65%) after dialysis.  It was noted that there are two peaks of Trx when using ion exchange-type DEAE-cellulose for the product of gel filtration column. The approximately molecular weight of the isolated Trx using gel filtration chromatography (G-50) and electrophoresis technique type SDS-PAGE were (12305 + 190) Dalton and 12461 Dalton respectively . The results showed that inhibited and activated effects of drugs on Trx working. The results showed that inhibited to 44.59% for paracetamol, dexathamazone, diphenhydramine-HCl, theophlline anhydrous,  pseudoephedrine, ceramide and caffeine anhydrous, but metronidazole and chlorpheniramine malate have activated effect to 110.8% and 21.62 respectively.