A. D. Iaremchuk, M. Tukalo, S. Egorova, A. Konovalenko, G. Matsuka
{"title":"[Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27].","authors":"A. D. Iaremchuk, M. Tukalo, S. Egorova, A. Konovalenko, G. Matsuka","doi":"10.7124/BC.0000EA","DOIUrl":null,"url":null,"abstract":"A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.","PeriodicalId":23403,"journal":{"name":"Ukrainskii biokhimicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1989-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ukrainskii biokhimicheskii zhurnal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7124/BC.0000EA","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
