Determination of Carbapenem-Resistant Enterobacteriaceae from Chicken Meat by Advanced Modified ISO 21528-1:2017 Method

Abstract

Antibiotic resistance is critical today, and carbapenem-resistant Enterobacteriaceae (CRE) are the current reflection of this threat in terms of public health. Our study aimed to investigate the effectiveness of a known ISO method used to detect of CREs in chicken meat sold in the Aksaray Region. A total of 150 chicken samples (50 drumsticks, 50 breasts, and 50 wings) were analyzed in terms of CRE by modifying the ISO 21528-1:2017 method. For this purpose, meropenem and ertapenem powders were added into buffered peptone water and Violet Red Bile Glucose Agar at the resistance levels determined in EUCAST. At the same time, target DNA extraction was performed from all samples with a tissue isolation kit (Hybrigen) and stored for PCR to support and strengthen our results to compare the cultural method's results. According to the results of the cultural procedure, the existing flora of chicken meats was suppressed by antibiotic supplements, and no suspicious colonies were detected. Likewise, CRE was not detected in DNA samples obtained from 150 chicken meat samples. Carbapenem resistance, described as the last fortress, is today's significant public health problem. According to our results, CRE was not isolated in 150 chicken meat samples offered for sale in our region.