The Influence of Technical Factors on the NBT Test

Bengt Björksténm
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引用次数: 3

Abstract

The results of NBT tests were found to vary depending on how the tests were performed. Heparin increased the proportion of NBT positive neutrophils and the lowest possible concentration should be used. Blood samples for the NBT test could be stored for 8 h at 4° C with only a minor effect on the test results. The incubation time was crucial and 25 min in a 37° C water bath was adopted as standard procedure.
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技术因素对NBT测试的影响
发现NBT测试的结果因执行测试的方式而异。肝素增加了NBT阳性中性粒细胞的比例,应使用尽可能低的浓度。用于NBT测试的血液样本可以在4°C下保存8小时,对测试结果只有轻微影响。孵育时间至关重要,标准程序为37°C水浴25 min。
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