Enzymatic production Of DNA oligos

Abstract

If DNA nanotechnology is to become a viable technology for therapeutics, diagnostics or life science research, the component materials mostly single stranded DNA will have to be produced cheaply, abundantly and with very high quality. Prior experiments demonstrated the production of clonal template through perfectly controlled relative stoichiometric ratios in a highly scalable enzymatic process called the MOSIC method (Monoclonal StoichiometrIC). To assess whether the technique for the production of oligos enzymatically could be useful in more applications in DNA nanotechnology, a pseudogene coding a crystal template was produced using helper phages in this project. This method enables the production of such single stranded DNA with high yield, low cost and easily scalable method.