Biological fabrication and characterization of Silver nanoparticles using Gymnemasylvestre and its medical application

Abstract

Background: The present study was aimed to green synthesis and characterization of silver nanoparticles (AgNPs) from Gymnema sylvestre, which was evaluated for their antimicrobial activity. Materials and Methods: The synthesized NPs were characterized by various analytical techniques such as ultraviolet–visible (UV–VIS) spectroscopy, Fourier transform-infrared (FT-IR), X-ray diffraction, dynamic light scattering (DLS), and transmission electron microscopy (TEM). It was confirmed through the UV–Vis spectrophotometer; corresponding peaks were identified at 424 nm. The green synthesized AgNPs were characterized by FT-IR studies to reveal the functional group attributed to the formation of AgNPs. Morphological size of AgNPs was 20 nm detect through characterization by DLS and TEM. Results: The green synthesized AgNPs showed vigorous antimicrobial activity against human pathogenic bacterial strains such as Enterobacter cloacae, Staphylococcus, hemolytic Staphylococcus petrasii subs. pragensis Bacillus cereus, and Staphylococcus aureus. Conclusion: These biosynthesized AgNPs were then used to demonstrate antimicrobial activity against a pathogen bacterium. The antibacterial activity of AgNP was clearly from the zone of inhibition. At concentrations (20 μg/ml–50μg/ml), the AgNP showed a clear zone of inhibition.