Using DNA-Logging to Determine Inflow Profile in Horizontal Wells

Arsenii Stanislavovich Posdyshev, P. Shelyakin, Nurislam Shaikhutdinov, A. A. Popov, M. Logacheva, M. Tutukina, M. Gelfand
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Abstract

The purpose of this work is to adapt and apply Next Generation Sequencing methods in oil and gas well field studies. Relatively recent NGS methods provide a description of a geological formation by analyzing millions of DNA sequences and represent an entirely new way to obtain information about oil and gas reservoirs and the composition of their fluids, which could significantly change the approach to exploration and field development. We present the results of pilot work to determine the inflow profile in a horizontal well based on DNA markers. The technology is based on the comparison of bacterial DNA from drill cuttings obtained while drilling with DNA from microorganisms of fluids obtained during production at the wellhead. Because of their high selectivity, individual microbes live only under certain conditions (salinity, oil saturation, temperature) and can be used as unique natural biomarkers. The comparison of DNA samples of drilling cutting and produced fluid allows for identification of the segment of the horizontal well from which the main flow comes, as well as identifying the type of incoming fluid (water, oil, gas) without stopping the operation process and without conducting expensive downhole operations. As a result of these studies, the microbial communities of the oil-bearing sands and formation fluids of the Cretaceous deposits (group BS) in Western Siberia were identified, and the relative numerical ratio of microorganisms in the formations was determined. It was shown that the microbiome diversity changes with depth, and depends on the lithological composition, and sequencing data obtained from cuttings samples correlate with data from wellhead samples of produced fluid. Thus, the practical applicability of DNA sequencing for solving field problems in oil and gas field development, in particular for determining the inflow profile in horizontal wells, was confirmed.
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利用dna测井技术确定水平井流入剖面
这项工作的目的是适应和应用下一代测序方法在油气井现场研究。相对较新的NGS方法通过分析数百万个DNA序列来描述地质构造,代表了一种获取油气储层及其流体组成信息的全新方法,这可能会显著改变勘探和油田开发的方法。我们介绍了基于DNA标记确定水平井流入剖面的试点工作结果。该技术的基础是将钻井时获得的钻屑中的细菌DNA与井口生产过程中获得的流体中的微生物DNA进行比较。由于它们的高选择性,单个微生物只能在一定的条件下(盐度、含油饱和度、温度)生存,并且可以作为独特的天然生物标志物。通过对钻井切削液和产出液的DNA样本进行比较,可以在不停止作业过程和不进行昂贵的井下作业的情况下,识别出水平井中主要流体的来源,以及识别进入流体(水、油、气)的类型。在此基础上,对西伯利亚西部白垩系BS组油砂和地层流体中的微生物群落进行了识别,并确定了地层中微生物的相对数值比。结果表明,微生物组的多样性随深度而变化,并取决于岩性组成,从岩屑样品中获得的测序数据与井口采出液样品的数据具有相关性。因此,DNA测序在解决油气田开发中的现场问题,特别是在确定水平井流入剖面方面的实际适用性得到了证实。
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