Production Of Protease By Submerged Fermentation Using Rhizopus Microsporus Var Oligospous

L. Sarao, M. Arora, V. Sehgal, S. Bhatia
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引用次数: 6

Abstract

Production of protease via submerged fermentation using Rhizopus microsporus var oligospous was studied using shake flask cultures. After 96 h of growth on a BOD shaker revolving at 150 rpm, protease was partially purified using the isopropanol. Factors affecting the enzyme production viz incubation temperature, initial pH of the shake flask medium. and inoculum size were optimized. Protease having the maximum enzyme activity of 521.739 IU was obtained at an incubation temperature of 30oC, an initial pH of the basal medium as 5.5 and an inoculum size of to 1x10 spores ml of Tween-80. Protease deactivated at 80 oC.
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利用少穗小孢子根霉深层发酵生产蛋白酶的研究
用摇瓶培养法研究了小孢子根霉寡孢子根霉深层发酵生产蛋白酶的工艺。在150转/分的BOD摇床上生长96小时后,用异丙醇部分纯化蛋白酶。影响产酶的因素有孵育温度、摇瓶培养基的初始pH。并对接种量进行优化。在培养温度为30℃,基础培养基初始pH为5.5,接种量为1 × 10孢子ml的Tween-80的条件下,获得酶活性最高的蛋白酶为521.739 IU。蛋白酶在80℃时失活。
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