Antidiabetic actions of aqueous bark extract of Swertia chirayita on insulin secretion, cellular glucose uptake and protein glycation

H. Thomson, O. Ojo, P. Flatt, Y. Abdel-Wahab
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引用次数: 13

Abstract

Objective: There is renewed scientific interest in the potential of plant-derived agents for the treatment ofdiabetes mellitus. This study investigated the antidiabetic actions of Swertia chirayita, a plant used traditionally inthe management of diabetes. Materials and Methods: Insulin secretion from BRIN-DB11 cells was assessedin the absence or presence of plant extract and modulators of beta cell function. Glucose uptake was assessedusing 3T3-L1 cells while effects of the plant extract on protein glycation was assessed using model peptide.Insulin was measured by radioimmunoassay and intracellular calcium by FlexStation®. Results: S. chirayitasignificantly stimulated concentration-dependent insulin secretion from BRIN-BD11 cells. Its insulinotropiceffects were abolished in the absence of extracellular Ca2 + or by diazoxide and were significantly decreasedby verapamil and in beta cell depolarization with KCl. S. chirayita extracts evoked a 28-59% increase in basaland insulin-stimulated glucose uptake by 3T3-L1 cells. Protein glycation was significantly inhibited by S. chirayitain a dose-dependent manner. Conclusion: This study reveals that the antidiabetic actions of S. chirayitaaqueous bark extracts involves the stimulation of insulin secretion and enhancement of insulin action. Inhibitionof protein glycation may also help counter diabetic complications. These actions of S. chirayita may providenew opportunities for the treatment of diabetes.
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獐牙獐牙菜树皮水提物对胰岛素分泌、细胞葡萄糖摄取和蛋白糖化的作用
目的:研究植物源性药物治疗糖尿病的潜力。本研究探讨了獐牙獐牙菜的抗糖尿病作用,獐牙獐牙菜是一种传统上用于治疗糖尿病的植物。材料和方法:在不含或存在植物提取物和β细胞功能调节剂的情况下,评估BRIN-DB11细胞的胰岛素分泌。使用3T3-L1细胞评估葡萄糖摄取,使用模型肽评估植物提取物对蛋白质糖基化的影响。胰岛素用放射免疫法测定,细胞内钙用FlexStation®测定。结果:藏红花显著刺激BRIN-BD11细胞浓度依赖性胰岛素分泌。在缺乏细胞外Ca2 +或二氮氧化合物的情况下,其胰岛素作用被消除,维拉帕米和KCl对β细胞去极化的作用显著降低。山茱萸提取物诱导3T3-L1细胞基底区胰岛素刺激的葡萄糖摄取增加28-59%。蛋白糖基化作用明显受雪梨苷的抑制,且呈剂量依赖性。结论:本研究揭示了山茱萸树皮提取物的抗糖尿病作用可能与刺激胰岛素分泌和增强胰岛素作用有关。抑制蛋白糖化也可能有助于对抗糖尿病并发症。这些作用可能为糖尿病的治疗提供新的机会。
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