Molecular identification and characterization of turkey IFN-γ gene

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2001-12-01 DOI:10.1016/S1096-4959(01)00469-9

Chien C Loa, Ming K Hsieh, Ching C Wu, Tsang L Lin

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Abstract

The cDNAs of turkey and chicken interferon gamma (IFN-γ) were cloned and the functional activity of turkey and chicken IFN-γ was compared. The coding region of turkey IFN-γ gene encodes a predicted mature protein of 145 amino acids with a molecular weight at 16.8 kDa. Compared with type I IFN, the IFN-γ between turkey and chicken also had the same size and high degree of identity at the nucleotide (96.0%) and amino acid (96.4%) sequence. Turkey IFN-γ was cross-reactive with chicken cells. Both turkey and chicken IFN-γ could induce production of nitric oxide by turkey or chicken macrophages. Turkey IFN-γ also had similar degree of sensitivity to heat and pH 2.0 as chicken IFN-γ. The functional activity of both turkey and chicken IFN-γ could be neutralized by a monoclonal antibody specific to chicken IFN-γ to a similar extent. These results indicated that IFN-γ protein was cross-reactive between turkey and chicken.

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火鸡IFN-γ基因的分子鉴定与表征

克隆了火鸡和鸡干扰素γ (IFN-γ)的cdna，比较了火鸡和鸡干扰素γ的功能活性。火鸡IFN-γ基因编码区编码145个氨基酸的成熟蛋白，分子量为16.8 kDa。与I型IFN相比，火鸡和鸡之间的IFN-γ在核苷酸（96.0%）和氨基酸（96.4%）序列上具有相同的大小和高度的同源性。火鸡IFN-γ与鸡细胞有交叉反应。火鸡和鸡IFN-γ均能诱导火鸡或鸡巨噬细胞产生一氧化氮。火鸡IFN-γ对高温和pH值2.0的敏感性也与鸡IFN-γ相似。鸡IFN-γ特异性单克隆抗体可以在相似程度上中和火鸡和鸡的IFN-γ的功能活性。这些结果表明IFN-γ蛋白在火鸡和鸡之间存在交叉反应。

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来源期刊

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology 生物-动物学

CiteScore

4.60

自引率

4.50%

发文量

审稿时长

22 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part B: Biochemical and Molecular Biology (CBPB), focuses on biochemical physiology, primarily bioenergetics/energy metabolism, cell biology, cellular stress responses, enzymology, intermediary metabolism, macromolecular structure and function, gene regulation, evolutionary genetics. Most studies focus on biochemical or molecular analyses that have clear ramifications for physiological processes.