Mutation Detection via CRISPRCas9 Micro-fluid Chip

Abstract

In biology mutation is a change in the nucleotide sequences of the DNA of an organism mainly there are three types of mutation: point mutation, deletion and insertions. Once the mutation has been defined allele specific oligonucleotide hybridization, amplification, heteroduplex formation method referred to as a diagnostic method some advance technique like CRISPR cas9 system is using for selected mutagenesis. Using microfluid and CRISPR cas9 system we can detect a mutation. Let's say you have a DNA sample with fluorescent labeled from patient and you want to make sure that gene you are interested is in healthy gene. We can design a CRISPR to scan through DNA or find specific gene or mutation. The CRISPR scan the DNA if the CRISPR does not find targeted gene it does not bind to it its means that no fluorescence color appears under UV-light but its scan and find its target and this binding create a fluorescence signal its means that mutation can be occur in gene