{"title":"Methods of Detecting Meat Species in Food of Animal Origin","authors":"Lavinia-Maria Chiş, D. Vodnar","doi":"10.15835/buasvmcn-fst:2018.0009","DOIUrl":null,"url":null,"abstract":"An important factor in the detection of falsification is the control of the composition of the meat at each stage of manufacturing the product. The PCR method is based on the study of proteins and meat nucleic acids used in food for the detection of animal species. Another technique is the Elisa method that works on the principle of identification and measurement of the quantity of molecules in a sample. There are several types of Elisa to increase specificity due to differences in structure and sample characteristics. By comparing the two methods used to identify the processed meat product species, Real Time PCR had the highest prediction as results. However, the Elisa method is more time efficient and easier to use. Real Time PCR is effective in identifying processed meat products that require low detection. The Elisa Kit is useful because of the ease of use.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.3000,"publicationDate":"2018-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15835/buasvmcn-fst:2018.0009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 2
Abstract
An important factor in the detection of falsification is the control of the composition of the meat at each stage of manufacturing the product. The PCR method is based on the study of proteins and meat nucleic acids used in food for the detection of animal species. Another technique is the Elisa method that works on the principle of identification and measurement of the quantity of molecules in a sample. There are several types of Elisa to increase specificity due to differences in structure and sample characteristics. By comparing the two methods used to identify the processed meat product species, Real Time PCR had the highest prediction as results. However, the Elisa method is more time efficient and easier to use. Real Time PCR is effective in identifying processed meat products that require low detection. The Elisa Kit is useful because of the ease of use.
检测伪造的一个重要因素是在产品制造的每个阶段对肉类成分的控制。PCR方法是基于对食品中用于动物物种检测的蛋白质和肉类核酸的研究。另一种技术是Elisa法,它的工作原理是识别和测量样品中的分子数量。由于结构和样品特性的差异,有几种类型的Elisa可以提高特异性。通过比较两种用于肉制品品种鉴定的方法,Real Time PCR的预测结果最高。然而,Elisa法更省时,更容易使用。实时聚合酶链反应是有效的识别加工肉制品,需要低检测。Elisa试剂盒因易于使用而非常有用。