Effect of Aging on Testis Structure, GLUT2, MTOR Expression and Testosterone Level in Spermatogenesis of The Rats

Maiada Moustafa, B. Hassan
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Abstract

Background : Male ageing has been previously associated with declining sperm parameters, disrupted hormone secretion and increased time to pregnancy. Aging is a natural ongoing process characterized by morphological and structural degeneration and like other organs. Glucose is essential for testicular function; the uptake of carbohydrate-derived glucose by cells is mediated by glucose transporters (GLUTs). MTOR is also involved in the maintenance and restructuring of the blood-testis barrier (BTB), a key event in the seminiferous epithelium cycle Aim of the work : In the present study, we aimed to investigate the relationship between aging and the expression of GLUT2 and MTOR in the testis, the quality of the spermatogenesis process as well as the testosterone level. Materials and methods: Eighteen rats were classified into the following experimental age groups, premature, mature and aged the rats were weighed and sacrificed by decapitation under a mild dose of anesthetic ether testicular tissue was existed for histological, histochemical, immunohistochemical and molecular techniques. Results: In the aged group, Hematoxylin and Eosin stain, showed a decreased number of sperms that were observed in the lumen of seminiferous tubules, weak PAS reaction in basement membranes moderate immunohistochemical reaction for GLUT2 and MTOR antibodies, also decreased level of testosterone hormone was observed. Conclusion : spermatogenesis process is affected badly by aging that was clear histologically in testicular tissue and the decreased level of testosterone hormone.
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衰老对大鼠睾丸结构、GLUT2、MTOR表达及睾丸激素水平的影响
背景:男性衰老以前与精子参数下降、激素分泌中断和怀孕时间延长有关。衰老是一种自然的持续过程,其特征是形态和结构的退化,就像其他器官一样。葡萄糖对睾丸功能至关重要;细胞对糖源性葡萄糖的摄取是由葡萄糖转运蛋白(GLUTs)介导的。MTOR还参与了血睾丸屏障(BTB)的维持和重建,这是精细胞上皮周期的一个关键事件。工作目的:在本研究中,我们旨在研究睾丸中GLUT2和MTOR的表达、精子发生过程的质量以及睾酮水平与衰老的关系。材料与方法:将18只大鼠分为早产、成熟和老年三组,称重后在小剂量麻醉醚下斩首处死,取睾丸组织进行组织学、组织化学、免疫组织化学和分子技术分析。结果:老龄组精小管腔内精子数量减少,基底膜PAS反应弱,GLUT2、MTOR抗体免疫组化反应中等,睾酮水平下降。结论:衰老对精子发生过程有明显影响,组织学上表现为睾丸组织衰老和睾丸激素水平下降。
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审稿时长
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