Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Mung Bean under Abiotic Stress and Cercospora canescens Infection

IF 0.8 4区 生物学 Q3 AGRONOMY Legume Research Pub Date : 2020-04-12 DOI:10.18805/lr-507
X. Ke, L. Yin, J. Xu, W. Sun, X. Xu, Y. Guo, Y. Zuo
{"title":"Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Mung Bean under Abiotic Stress and Cercospora canescens Infection","authors":"X. Ke, L. Yin, J. Xu, W. Sun, X. Xu, Y. Guo, Y. Zuo","doi":"10.18805/lr-507","DOIUrl":null,"url":null,"abstract":"The objective of the study was to identify suitable reference genes that can be used for quantitative real-time PCR (qPCR) analysis in mung bean (Vigna radiata). Therefore, 10 potential reference genes were selected and the results showed that ubiquitin-conjugating enzyme was suitable as reference under drought and pathogen infection stress; elongation factor 1-a was the most stable gene under waterlogging; and actin performed the best under saline stress. These selected reference genes were further confirmed by analysis of the expression profiles of catalase and peroxidase under waterlogging. Our results will contribute to the improvement of the accuracy of gene expression evaluation in mung bean.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"32 1","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2020-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Legume Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.18805/lr-507","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 1

Abstract

The objective of the study was to identify suitable reference genes that can be used for quantitative real-time PCR (qPCR) analysis in mung bean (Vigna radiata). Therefore, 10 potential reference genes were selected and the results showed that ubiquitin-conjugating enzyme was suitable as reference under drought and pathogen infection stress; elongation factor 1-a was the most stable gene under waterlogging; and actin performed the best under saline stress. These selected reference genes were further confirmed by analysis of the expression profiles of catalase and peroxidase under waterlogging. Our results will contribute to the improvement of the accuracy of gene expression evaluation in mung bean.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
实时荧光定量PCR分析绿豆在非生物胁迫和黑尾孢感染下基因表达的参考基因
本研究的目的是寻找适合于绿豆(Vigna radiata)实时荧光定量PCR (qPCR)分析的内参基因。结果表明,在干旱和病原菌侵染胁迫下,泛素偶联酶适宜作为内参基因;涝渍条件下伸长因子1-a最稳定;肌动蛋白在生理盐水胁迫下表现最好。这些内参基因通过内涝条件下过氧化氢酶和过氧化物酶的表达谱分析得到进一步证实。本研究结果将有助于提高绿豆基因表达评价的准确性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Legume Research
Legume Research AGRONOMY-
CiteScore
1.30
自引率
37.50%
发文量
256
审稿时长
6-12 weeks
期刊介绍: Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.
期刊最新文献
Effect of Different Irrigation Regimes on the Yield and Qualitative Characteristics of Different Cultivars of Soybean (Glycine max L.) in Jiroft Productivity and Econometric Analysis of Leguminous Monkey-nut (Arachis hypogaea L.) as Influenced by Growth Retardant Long-term Experiment on Fennel-cluster Bean Cropping System as Affected by Production Systems: A Strategy for Improving Yield, Quality and Soil Fertility Response of IWM Strategies on Growth, Nodulation, Productivity and Profitability in Fenugreek (Trigonella foenum-graecum L.) under Haryana Conditions Influence of Morphological and Biochemical Parameters for Infection of Mungbean Yellow Mosaic Virus and Bemisia tabaci in Mungbean and Urdbean
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1