{"title":"Self-assembled silanes and the thiol functionalization of surfaces","authors":"M. McGovern, M. Thompson","doi":"10.1039/A807021K","DOIUrl":null,"url":null,"abstract":"The relative thickness and coverage of the silanes, mercaptopropyltrimethoxysilane and 1-(trifluoroacetato-)-11-trichlorosilyl-undecane subsequent to reaction on silicon surfaces has been assessed by X-ray photoelectron spectroscopic analysis. Use of the probe, N-pentafluorophenyl-N′-iodoacetyl hydrazide, allows an estimation of the availability of thiol groups on the substrate surface following reaction in air and in O2-free conditions. Although the trimethoxysilane generates multilayer structures, it is only capable of attaching 3.5 times less probe than the fluorine-containing silane. The results of these experiments confirm that 1-(trifluoroacetato-)-11-trichlorosilyl-undecane is a much more effective linking agent for the immobilization of biomolecules to substrate surfaces.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"74 1","pages":"391-393"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/A807021K","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
The relative thickness and coverage of the silanes, mercaptopropyltrimethoxysilane and 1-(trifluoroacetato-)-11-trichlorosilyl-undecane subsequent to reaction on silicon surfaces has been assessed by X-ray photoelectron spectroscopic analysis. Use of the probe, N-pentafluorophenyl-N′-iodoacetyl hydrazide, allows an estimation of the availability of thiol groups on the substrate surface following reaction in air and in O2-free conditions. Although the trimethoxysilane generates multilayer structures, it is only capable of attaching 3.5 times less probe than the fluorine-containing silane. The results of these experiments confirm that 1-(trifluoroacetato-)-11-trichlorosilyl-undecane is a much more effective linking agent for the immobilization of biomolecules to substrate surfaces.
用x射线光电子能谱分析评价了硅烷、巯基丙基三甲氧基硅烷和1-(三氟乙酸-)-11-三氯硅十一烷在硅表面反应后的相对厚度和覆盖范围。使用探针n -五氟苯基- n ' -碘乙酰肼,可以估计在空气和无o2条件下反应后底物表面巯基的可用性。虽然三甲氧基硅烷可以产生多层结构,但其附着探针的数量仅为含氟硅烷的3.5倍。这些实验结果证实,1-(三氟乙酸-)-11-三氯硅十一烷是一种更有效的连接剂,用于固定化生物分子到底物表面。