Isolation and identification of some uncommon bacterial species isolated from different clinical sample

Abstract

There are many opportunistic bacterial species that are uncommon and infrequently exist inclinical specimen, most of them are difficult to routine identification, even some of them arepoorly documented in clinical specimen. also had no less role in the coordinates of the diseasethan common bacterial species. Six hundred and fifty samples were collected from patientsattending to some hospitals in Sulaimanya City and Kalar General Hospital during the periodfrom October 2015 to November 2016.Samples were firstly cultured on different media in order to isolate and identify bacterialisolates according to cultural characteristics, morphological features and biochemical reactions inaddition to Vitek 2 system for identifying uncommon and infrequent isolates. The identificationand susceptibility test were performed in Kalar General Hospital. Isolated 286(44%) bacterialstrains from different clinical samples, 125 of them were identified by Vitek 2 automatedsystem, while 23(8%) of isolates were considered as uncommon bacterial species.The antimicrobial susceptibility of uncommon isolates, showed significant variation againsttwenty four antibiotics. Four isolates; Acinatobacter baumanii, Acinatobacter calcoaceticus,Enterobacter ludwigii and Gemella sanguinis were resistant to all antibiotics. WhereasAerococcus urinae, (Citrobacter freundii and Alloiococcus otitis), (Morganella morganii,Sphingomonas paucimobilis, Pantoea agglomerans, Streptococcus sobrinus and Kocuria rosean.2), ( Ochrobactrum anthropi n.1 and Kocura kristinae), (Ochrobactrum anthropi n.2 andKocuria rosea n.1 ), (Pseudomonas stutzeri, Pseudomonas fluorescens and Citrobacter koseri ),(Aeromonas salmonicida and Micrococcus luteus n.1) and Micrococcus luteus (n.2) resistant tothe antibiotics as these percentages 95.8%, 91.6%, 87.5%, 83.3%, 79.1%, 70.8%, 66.6% and62.5%, respectively.