Evaluation of salt-tolerant Azospirillum spp and its role in improvement of wheat growth parameter

I. El-Akhdar, M. El-sheekh, N. Allam, F. Kamal, R. Abou-Shanab, C. Staehelin
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引用次数: 6

Abstract

Eighteen isolates of Azospirillum were collected from different sites at Kafrelsheikh Governorate. The isolates were cultivated on nitrogen-free malate (NFM) medium supplemented with different NaCl gl-1 concentrations (Zero, 0.7 and 1.5%) and tryptophan to study the salt tolerant in vitro and indol acetic acid production. Pots were used to evaluate the most salt-tolerant and efficient species and soils of this experiment had artificially salinized with different salinity levels. The most salt-tolerant A10 and A11 species were genetically identified by 16S rRNA sequencing and the results revealed to be closest matched at 99% A. lipoferum strains and selected to inoculate wheat plants. The investigated A. lipoferum species had variable divergence growth at different salinity levels. Actually, increased salinity had deleterious effect on IAA production. In addition, salinity had deleterious effect on dry weights of plants, number and dry weight of branches, total chlorophyll, sodium and potassium% with increasing salinity levels. But, inoculation with associative nitrogen fixation A. lipoferum isolates enhanced the previous parameters. Thus, inoculation with the salt-tolerant A.lipoferum isolates decreased the deleterious effect of salt stress on wheat plants.
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耐盐氮螺旋菌的鉴定及其对小麦生长参数的改善作用
在Kafrelsheikh省不同地点采集到18株氮螺旋菌分离株。在无氮苹果酸(NFM)培养基中添加不同NaCl(0、0.7和1.5%)和色氨酸,研究菌株的体外耐盐性和吲哚乙酸产量。本试验采用盆栽法评价最耐盐高效品种,并对土壤进行不同盐度的人工盐渍化处理。通过16S rRNA测序对耐盐性最强的A10和A11进行了遗传鉴定,结果显示,其与99%的A. lipoferum菌株最匹配,并被选择用于接种小麦植株。不同盐度条件下,脂藻属植物的发散生长是不同的。实际上,盐度的增加对IAA的产生是有害的。此外,随着盐度的增加,植株干重、分枝数和干重、总叶绿素、钠、钾%均有不利影响。而接种联合固氮A. lipoferum菌株可提高上述参数。由此可见,接种耐盐脂孢单胞菌可降低盐胁迫对小麦植株的危害。
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