Investigating the relationship between C-159T polymorphism in CD14 gene promoter and disease severity in 2019 corona virus patients

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Abstract

Introduction: Considering the effectiveness of polymorphisms in the occurrence of respiratory diseases, it seems that these genetic variations in viral and microbial receptors can be effective in the occurrence and progression of the disease. The present study aimed to assess the relationship between the C-159T polymorphism in the CD14 gene promoter and the severity of the disease in Covid-19 patients during 2020-2021. Method: The subjects were randomly selected from subjects referred for polymerase chain reaction (PCR) and studied in five groups: healthy, outpatient, non-critically ill hospitalized, critically ill hospitalized, and deceased patients. The peripheral blood (3ml) was obtained from all patients and DNA was extracted. Thereafter, C-159T polymorphism in the CD14 gene promoter was determined by PCR-restriction fragment length polymorphism (RFLP) method. The genotype frequency was determined in terms of heterozygosity, homozygosity, and mutant regarding polymorphism. The data were analyzed in SPSS software (version 21) (P<0.05). Results: The results pointed out that the most common C-159T polymorphism was the CT polymorphism, which was observed in 107 (51.0%) subjects and was significantly higher than other polymorphisms in all PCR- and PCR+ participants. This difference was not significant for deceased people. Conclusion: According to the results pointing out that the C-159T polymorphism is effective in causing the disease, as well as the significant relationship between the TT and CT genotypes with the coronavirus, the probability of developing the disease is higher in T allele carriers. Therefore, it is suggested that CT polymorphism in CD14 gene promoter is considered an important prognosis in coronavirus disease.
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研究2019冠状病毒患者CD14基因启动子C-159T多态性与疾病严重程度的关系
导读:考虑到多态性在呼吸道疾病发生中的有效性,似乎这些病毒和微生物受体的遗传变异在疾病的发生和进展中是有效的。本研究旨在评估2020-2021年期间Covid-19患者CD14基因启动子C-159T多态性与疾病严重程度之间的关系。方法:从聚合酶链反应(PCR)转诊对象中随机抽取受试者,分为健康患者、门诊患者、非危重住院患者、危重住院患者和死亡患者5组。所有患者取外周血3ml,提取DNA。随后,采用pcr -限制性片段长度多态性(RFLP)方法检测CD14基因启动子中C-159T的多态性。基因型频率根据杂合度、纯合度和多态性突变来确定。数据采用SPSS软件(第21版)进行分析(P<0.05)。结果:结果指出,C-159T最常见的多态性为CT多态性,107例(51.0%)受试者中存在CT多态性,在所有PCR-和PCR+受试者中均显著高于其他多态性。这种差异对死者来说并不显著。结论:根据C-159T多态性有效致病的结果,以及TT和CT基因型与冠状病毒的显著相关性,T等位基因携带者发病的概率更高。因此,提示CD14基因启动子的CT多态性被认为是冠状病毒病的重要预后因素。
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