Interaction Forces between BSA Adsorbed Layer on Amino-Thiolated Gold Surfaces

D. Hiraiwa, K. Torigoe, Tomokazu Yoshimura, K. Esumi
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Abstract

Direct measurement of interaction forces between bovine serum albumin (BSA) adsorbed layers on amino-thiolated gold surfaces has been carried out using colloidal probe atomic force microscopy (AFM). The substrate was prepared via self-assembly of 2-aminoethanethiol on gold in aqueous media. In the absence of BSA, an electric double layer interaction was observed, resulting from protonation of the primary amine groups on the gold surface. Similar electric double layer repulsions were seen in the presence of BSA over a whole range of BSA concentration investigated. These repulsive interactions were found to be dependent on the BSA concentration, that is, the magnitude of the repulsion increases with the BSA concentration. This phenomenon is attributed to the difference in the adsorbed amount of BSA on the amino-thiolated gold surface, which was confirmed using a 27 MHz quartz crystal microbalance (QCM).
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氨基硫化金表面上BSA吸附层间的相互作用力
利用胶体探针原子力显微镜(AFM)直接测量了氨基硫化金表面上牛血清白蛋白(BSA)吸附层之间的相互作用力。在水介质中,通过在金上自组装2-氨基乙硫醇制备底物。在没有牛血清蛋白的情况下,由于金表面的伯胺基团质子化,观察到电双层相互作用。在研究的整个BSA浓度范围内,发现了类似的双电层排斥现象。发现这些排斥相互作用依赖于BSA浓度,即斥力的大小随着BSA浓度的增加而增加。这一现象是由于氨基硫化金表面上BSA吸附量的差异,使用27 MHz石英晶体微天平(QCM)证实了这一点。
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