Effects of Oxytocin on Glutamate Mediated Neurotoxicity in Neuroblastoma Cell Culture.

IF 9.1 2区物理与天体物理 Q1 ASTRONOMY & ASTROPHYSICS Space Science Reviews Pub Date : 2024-02-21 eCollection Date: 2024-01-01 DOI:10.29399/npa.28377

Börte Gürbüz Özgür, Kamil Vural, Mehmet İbrahim Tuğlu

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Abstract

Introduction: We aimed to investigate the effects of oxytocin on neurite growth, cell viability, cell proliferation and apoptosis to demonstrate its neuroprotective effect on glutamate induced neurotoxicity in human neuroblastoma SH-SY5Y cell culture.

Method: The effect of oxytocin on the toxic effects of glutamate in human neuroblastoma SH-SY5Y cell line with the Neurotoxicity Screening Test (NTT), apoptotic effects by Terminal Transferase dUTP Nick End Labeling (TUNEL) method and cell viability test by 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) method. In the NTT test; Neurotoxicity was induced by adding glutamate at a concentration of 32 μM to the cell culture. Oxytocin was added at 1, 3, 10, 30 and 100 μM concentrations and its effect on neurite elongation was investigated. It was demonstrated by TUNEL method that application of glutamate caused apoptosis. Afterwards, when glutamate and different doses of oxytocin were given, antiapoptotic effect was evaluated with the apoptotic index.

Results: Glutamate was found to have a dose-dependent neurotoxic effect and reduced neurite elongation by 50% at a concentration of 32 μM. It was shown that the inhibition of neurite elongation caused by glutamate decreased in a dose-dependent manner by applying oxytocin. Especially oxytocin was found to significantly reduce neurite inhibition and show a neuroprotective effect starting from 10 μM concentrations. The concentration at which glutamate reduces cell proliferation by 50% was determined as 54 μM in MTT. Subsequently, it was observed that the adverse effect of glutamate on cell proliferation significantly decreased with oxytocin administration, depending on the dose.

Conclusion: It was found that different concentrations of glutamate have a significant toxic effect on cell proliferation and viability, glutamate inhibits neurite elongation in a dose-dependent manner; oxytocin reduces neurite inhibition caused by glutamate, has a neuroprotective effect, increases cell viability and has antiapoptotic effects.

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催产素对神经母细胞瘤细胞培养中谷氨酸介导的神经毒性的影响

简介：我们旨在研究催产素对神经元生长、细胞活力、细胞增殖和细胞凋亡的影响，以证明其对人神经母细胞瘤SH-SY5Y细胞培养中谷氨酸诱导的神经毒性的神经保护作用：用神经毒性筛选试验（NTT）检测催产素对人的神经母细胞瘤SH-SY5Y细胞株谷氨酸盐毒性作用的影响，用末端转移酶dUTP尼克末端标记（TUNEL）法检测细胞凋亡作用，用3-(4.5-二甲基噻唑-2-基)-2.5-二苯基溴化四氮唑（MTT）法检测细胞活力。在 NTT 试验中，向细胞培养物中加入浓度为 32 μM 的谷氨酸诱导神经毒性。催产素的浓度分别为 1、3、10、30 和 100 μM，研究其对神经元伸长的影响。用 TUNEL 法证明，谷氨酸会导致细胞凋亡。之后，给予谷氨酸和不同剂量的催产素，用凋亡指数评估抗凋亡效果：结果：谷氨酸具有剂量依赖性神经毒性作用，浓度为 32 μM 时神经元伸长减少 50%。结果表明，谷氨酸对神经元伸长的抑制与催产素的剂量有关。尤其是催产素，从 10 μM 浓度开始就能显著减少神经元抑制，并显示出神经保护作用。在 MTT 中，谷氨酸可使细胞增殖减少 50%的浓度被确定为 54 μM。随后观察到，谷氨酸钠对细胞增殖的不利影响随着催产素剂量的增加而明显降低：结论：研究发现，不同浓度的谷氨酸盐对细胞的增殖和活力有明显的毒性作用，谷氨酸盐以剂量依赖的方式抑制神经元的伸长；催产素可减少谷氨酸盐对神经元的抑制，具有神经保护作用，提高细胞活力，并有抗细胞凋亡的作用。

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来源期刊

Space Science Reviews 地学天文-天文与天体物理

CiteScore

19.70

自引率

3.90%

发文量

审稿时长

4-8 weeks

期刊介绍： Space Science Reviews (SSRv) stands as an international journal dedicated to scientific space research, offering a contemporary synthesis across various branches of space exploration. Emphasizing scientific outcomes and instruments, SSRv spans astrophysics, physics of planetary systems, solar physics, and the physics of magnetospheres & interplanetary matter. Beyond Topical Collections and invited Review Articles, Space Science Reviews welcomes unsolicited Review Articles and Special Communications. The latter encompass papers related to a prior topical volume/collection, report-type papers, or timely contributions addressing a robust combination of space science and technology. These papers succinctly summarize both the science and technology aspects of instruments or missions in a single publication.

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