Excretory/secretory antigens (ES) from in-vitro cultures of Taenia crassiceps cysticerci, and use of an anti-ES monoclonal antibody for antigen detection in samples of cerebrospinal fluid from patients with neurocysticercosis

Annals of Tropical Medicine & Parasitology Pub Date : 2002-06-01 DOI:10.1179/000349802125001140

N. M. Espíndola, A. Vaz, A. Pardini, I. Fernandes

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引用次数: 35

Abstract

Abstract Antigens were obtained from cysticerci of the ORF strain of Taenia crassiceps, by culture of cysts in protein-free hybridoma medium (PFHM). Budding of new vesicles was observed after 24-48h. Excretory/secretory (ES) antigens (peptides of <20kDa) were recovered in the medium after culture for 48h. SDS-PAGE analysis of vesicular-fluid (VF) antigens (obtained by rupturing T. crassiceps cysticerci in PFHM) and the ES antigens indicated partial homology between the two preparations. ES peptides of 18- and 14-kDa were recognized by polyclonal antibodies produced in rabbits immunized either with the VF antigens or with a total-antigen preparation of T. solium cysticerci. Antibodies present in samples of serum or cerebrospinal fluid (CSF) from patients with neurocysticercosis also reacted with ES peptides. An anti-ES monoclonal antibody detected antigens in the CSF from 10 patients with neurocysticercosis, showing the antigenic homology of the ES antigens with those of T. solium cysticerci in human infections.

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体外培养的猪带绦虫囊尾蚴的排泄/分泌抗原(ES)，以及抗ES单克隆抗体在神经囊虫病患者脑脊液样本中的抗原检测

摘要采用无蛋白杂杂瘤培养基(PFHM)培养的方法，从带绦虫ORF株囊尾蚴中获得抗原。24-48h后观察新泡的萌发。排泄/分泌(ES)抗原(<20kDa的肽)培养48h后在培养基中恢复。囊液(VF)抗原与ES抗原的SDS-PAGE分析显示两者具有部分同源性。18- kda和14-kDa的ES肽被免疫VF抗原或全抗原制备的囊尾蚴绦虫制备的多克隆抗体所识别。神经囊虫患者血清或脑脊液(CSF)样本中存在的抗体也与ES肽反应。抗ES单克隆抗体检测了10例神经囊虫病患者脑脊液中的抗原，显示ES抗原与人感染猪囊尾蚴的抗原同源性。

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