Lin Bo-wen, Xu Zhong-shi, X. De-ming, LU X iao-fu, Yang Da-zhi
{"title":"Establishment of an Animal Model for Experimental Study of Bone Nonunion","authors":"Lin Bo-wen, Xu Zhong-shi, X. De-ming, LU X iao-fu, Yang Da-zhi","doi":"10.13471/J","DOIUrl":null,"url":null,"abstract":"目的：对人类组织相容性抗原（Ｈｕｍａｎ Ｌｅｕｃｏｃｙｔｅ Ａｎｔｉｇｅｎ Ｓｙｓｔｅｍ）Ⅱ类进行高分辨基因分析。方法：用多重ＰＣＲ和等位基因特异性ＰＣＲ技术相结合＾［１，２］，建立多重等位基因特异性。结果：２４对引物成功在分辨４３份ＤＮＡ的ＨＬＡ－ＤＲＢ１、Ｂ３、Ｂ４、Ｂ５＾＊５１个等位基因和ＱＤＢ１＾＊１５个等位基因的４位数字特异性，识别基因片段长度为２８１ｂｐｏ。结论：多重等位基因特异性ＰＣ","PeriodicalId":17184,"journal":{"name":"Journal of Sun Yat-sen University","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2002-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Sun Yat-sen University","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.13471/J","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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