Biosynthesis of Silver Nanoparticle by Amphiroa anceps (Lamarck) Decaisne and Its Biomedical and Ecological Implications

Abstract

The present study synthesized silver nanoparticles by using seaweed extract and tested it against clinical bacterial pathogens and also with seeds to assess its friendly potential to germination. The biosynthesized Silver Nanoparticles was characterized with UV-Visible Spectrophotometer, Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS) and X-ray Diffraction study. Antibacterial activity was evaluated using agar disc diffusion and the seed germination test was done with standard protocol. The nanoparticles exhibited highest antibacterial activity against Escherichia coli (0.95 ± 0.057 cm), followed by Klebsiella pneumoniae (0.63 ± 0.05 cm), as compared to antibiotic Chloramphenicol (5 mg/ml). The per cent seed germination was 75% and 80% respectively in Abelmoschus esculentus and Raphanus sativus var. longipinnatus treated with nanoparticles, while it was only in a range of 20 to 40% in control seeds treated with normal water, seaweed extract and seaweed liquid bio-fertilizer. The antibacterial activity of the biosynthesized Silver Nanoparticle is needed more detailed investigation for its biomedical application. The potential of bio-nano-fertilizer is to be assessed for the whole plant life cycle up to quality and quantity of vegetable yield.