Aptamers against the Zika virus NS1 protein, for a serological diagnostic assay development

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE OF COMPUTATIONAL METHODS IN SCIENCES AND ENGINEERING 2019 (ICCMSE-2019) Pub Date : 2019-12-10 DOI:10.1063/1.5138061

L. Morais, A. Argondizzo, Dílson Silva, S. Missailidis

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引用次数: 1

Abstract

Zika virus (ZIKV) belongs to the Flaviviridae family and its recent outbreak and association with human diseases (e.g. neurological disorders and microcephaly in babies) have raised global health concerns. Its differentiation from Dengue in accurate diagnostic assays are of great importance. The viral NS1 protein is a secreted protein and can be an ideal target for diagnostic assay development. We have thus selected NS1 as target for aptamer selection and study. Single-stranded DNA oligonucleotides aptamers have been previously selected for this target, and here we present the study of such aptamers by fluorescent spectroscopy, CD and ELISA in order to characterize their interaction and select the best candidate for further future assay development.Zika virus (ZIKV) belongs to the Flaviviridae family and its recent outbreak and association with human diseases (e.g. neurological disorders and microcephaly in babies) have raised global health concerns. Its differentiation from Dengue in accurate diagnostic assays are of great importance. The viral NS1 protein is a secreted protein and can be an ideal target for diagnostic assay development. We have thus selected NS1 as target for aptamer selection and study. Single-stranded DNA oligonucleotides aptamers have been previously selected for this target, and here we present the study of such aptamers by fluorescent spectroscopy, CD and ELISA in order to characterize their interaction and select the best candidate for further future assay development.

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针对寨卡病毒NS1蛋白的适配体，用于血清学诊断检测的开发

寨卡病毒(ZIKV)属于黄病毒科，其最近的暴发及其与人类疾病(如神经系统疾病和婴儿小头畸形)的关联已引起全球卫生关注。其与登革热的鉴别诊断具有重要意义。病毒NS1蛋白是一种分泌蛋白，可以作为诊断检测开发的理想靶点。因此，我们选择NS1作为适体筛选和研究的靶点。单链DNA寡核苷酸适配体之前已经被选择用于这个靶点，在这里，我们通过荧光光谱、CD和ELISA对这些适配体进行研究，以表征它们的相互作用，并为进一步的检测开发选择最佳的候选体。寨卡病毒(ZIKV)属于黄病毒科，其最近的暴发及其与人类疾病(如神经系统疾病和婴儿小头畸形)的关联已引起全球卫生关注。其与登革热的鉴别诊断具有重要意义。病毒NS1蛋白是一种分泌蛋白，可以作为诊断检测开发的理想靶点。因此，我们选择NS1作为适体筛选和研究的靶点。单链DNA寡核苷酸适配体之前已经被选择用于这个靶点，在这里，我们通过荧光光谱、CD和ELISA对这些适配体进行研究，以表征它们的相互作用，并为进一步的检测开发选择最佳的候选体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PROCEEDINGS OF THE INTERNATIONAL CONFERENCE OF COMPUTATIONAL METHODS IN SCIENCES AND ENGINEERING 2019 (ICCMSE-2019)

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