Evaluating the Anti-Cancer Effect of Pomegranate Aqueous Extract of Gold Nanoparticles by Using Photodynamic Laser on Laboratory Mice.

Abstract

Background: This study evaluated the anticancer effects of gold nanoparticles (Au- NPs) generated by reducing hydrated gold metal ions in the presence of aqueous pomegranate peel extract. Aim of study: To test the possibility that Au-NPs generated from the pomegranate aqueous extract could be employed as an anti-cancer therapy. Materials and methods: The study aimed to evaluate Photodynamic therapy (PDT) using a laser at a wavelength of 532 nm with green gold particles as a photosensitizer in in-vivo experiment. Gold nanoparticles (Au-NPs) were produced by reducing hydrated gold metal ions in the presence of aqueous pomegranate peel extract. Using UV-visible spectroscopy, the absorption peak of the gold particles was found near 550- 592 nm. All diffraction peaks for 2θ at = 37.8227º at 43.7014º, 63.8103º and 77.1540º correspond to the (111), (200), (220), and (311) levels, respectively by X-ray diffraction(XRD) analysis, imaging confirmed Au-NP by Transmission electron microscopy (TEM), the nanoparticle size ranged between 19.96 ± 0.784 nm. Forty Mature Swiss females weighing 20–25 g and aged 6–8 weeks were used, the animals were placed in well-ventilated rooms. Results: The best exposure time to eradicate the tumor is with a duration of exposure of 30 minutes using the diode laser, as histological investigations indicated the development of anti-immunity with the same exposure period.