A Meta-analysis: Evaluating the Effect of METTL3/METTL14 on m6A Level Based on Knockdown Samples

The Fifth International Conference on Biological Information and Biomedical Engineering Pub Date : 2021-07-20 DOI:10.1145/3469678.3469715

Yu-xin Zhang, Yuxuan Wu, Di Zhen, Kunqi Chen, Jia Meng

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引用次数: 30

Abstract

N6-methyladenosine (m6A) is a dynamic modification regulated by the m6A enzymes prevalent on mRNA. The Mettl3 and mettl14 are two subunits of methyltransferase. Different studies have shown that knockout of the METL3 and MTEL14 genes has the potential to change methylation levels. We have therefore undertaken a meta-analysis study to assess the quality and quantity of available evidence. The focus of the research would on the effects of mRNA methylation level after the depletion of METTL3 or METTL14. R package “Metafor” was applied to output the model of random effect, the regarding risk ratios (RRs), and the 95% confidence intervals (CIs). Owing to the high heterogeneity of the overall data (I2>99%), some samples were selected and excluded to an acceptable level. Three published trials for METTL3 (Q = 12.85, I2 = 37.8%) and two for METTL4 (Q = 45.48, I2 = 82.9%) were qualified to review and analyze. Consequently, we successfully proved the correlation between the knockdown of METTL3/METTL14 and modification of methylation level.

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meta分析:基于敲低样本评价METTL3/METTL14对m6A水平的影响

n6 -甲基腺苷(m6A)是由mRNA上普遍存在的m6A酶调控的一种动态修饰。Mettl3和mettl14是甲基转移酶的两个亚基。不同的研究表明，敲除METL3和MTEL14基因有可能改变甲基化水平。因此，我们进行了一项荟萃分析研究，以评估现有证据的质量和数量。我们将重点研究METTL3或METTL14缺失后mRNA甲基化水平的影响。应用meta软件包输出随机效应模型、相关风险比(rr)和95%置信区间(ci)。由于整体数据的异质性较高(I2>99%)，部分样本被选择并排除到可接受的水平。已发表的3项METTL3试验(Q = 12.85, I2 = 37.8%)和2项METTL4试验(Q = 45.48, I2 = 82.9%)有资格进行评价和分析。因此，我们成功地证明了METTL3/METTL14的敲低与甲基化水平的修饰之间的相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Fifth International Conference on Biological Information and Biomedical Engineering

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