Nutzung cellulosehaltiger Abprodukte bei der Biosynthese von Cellulasen

D. G. Pobedimskij, I. E. Mukmenev, V. I. Savdur, A. G. Liakumovic, S. I. Agadganjan
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Abstract

It was analysed the influence of different pretreatment methods of cellulosic materials to increase the productivity of the cellulase biosynthesis. The most effective pretreatment method for cotton cellulose is the thermomechanical destruction in a worm extruder (5–15 minutes) with hydrolysis catalysts. The enzymatic saccharification degree was increased about 4 fold. Utilizing a pretreated cellulose an increase of 70–120% in C1-activity was found besides their level was stabilized in higher values. It was demonstrated that enzymatic hydrolysis parameters would be applied as model for biochemical utilization of cellulosic materials in the biosynthesis of cellulases.
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利用废糖体生成素产生蜂巢组织
分析了纤维素原料不同预处理方法对提高纤维素酶生物合成效率的影响。棉纤维素最有效的预处理方法是用水解催化剂在蠕虫挤出机中(5-15分钟)进行热机械破坏。酶促糖化程度提高约4倍。经预处理后的纤维素的c1活性提高了70-120%,并稳定在较高的水平。结果表明,酶解参数可作为纤维素原料在纤维素酶生物合成中的生化利用模型。
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