Effects of acute triclosan exposure on gill and liver tissues of zebrafish (Danio rerio)

IF 0.9 4区环境科学与生态学 Q4 LIMNOLOGY Annales De Limnologie-international Journal of Limnology Pub Date : 2021-01-01 DOI:10.1051/LIMN/2021004

Sezgi Arman

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引用次数: 6

Abstract

Triclosan (TCS) is a broad-spectrum antimicrobial agent found in personal care, household, veterinary, medical, and industrial products. TCS has been detected in soil, sediment, and surface water. In the current work, the effects of acute exposure to TCS on gill and liver tissues of zebrafish were evaluated. Adult zebrafish were treated with sublethal concentrations of TCS (34, 85, and 170 μg/L) for 120 h. Gill and liver samples were removed and embedded in paraffin for histopathological investigations. TCS induced aneurysm, capillary dilation, lamellar disorganization, hyperplasia, epithelial lifting, and desquamation in the gills. Liver specimens showed sinusoidal dilation, congestion, vacuolization, hepatocellular degeneration, and necrosis. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed to observe the apoptotic effect of TCS. Accordingly, no apoptotic cells were detected in the specimens of 34 μg/L of TCS exposed gills, while the slides of 85 and 170 μg/L of TCS treated gills showed a high number of TUNEL-positive cells. In the liver, no TUNEL-positive cells were identified in 34 μg/L of TCS treated samples of zebrafish. 85 and 170 μg/L of TCS exposed livers showed few hepatic parenchymal cells undergoing apoptosis.

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急性三氯生暴露对斑马鱼鳃和肝脏组织的影响

三氯生(TCS)是一种广谱抗菌剂，存在于个人护理、家用、兽医、医疗和工业产品中。TCS已在土壤、沉积物和地表水中检测到。本研究评估了急性暴露于TCS对斑马鱼鳃和肝脏组织的影响。用亚致死浓度的TCS(34、85和170 μg/L)处理成年斑马鱼120 h。取下鳃和肝脏标本，包埋于石蜡中进行组织病理学检查。TCS诱导动脉瘤、毛细血管扩张、板层解体、增生、上皮提升和鳃脱屑。肝标本显示肝窦扩张、充血、空泡化、肝细胞变性和坏死。采用末端脱氧核苷酸转移酶dUTP缺口末端标记法(TUNEL)观察TCS的凋亡作用。结果显示，34 μg/L TCS处理的鱼鳃标本中未检测到凋亡细胞，而85和170 μg/L TCS处理的鱼鳃载玻片中tunel阳性细胞较多。在34 μg/L TCS处理的斑马鱼肝脏中，未发现tunel阳性细胞。85和170 μg/L TCS暴露的肝脏中几乎没有肝实质细胞发生凋亡。

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来源期刊

Annales De Limnologie-international Journal of Limnology 地学-湖沼学

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Annales de Limnologie - International Journal of Limnology publishes papers on the ecology of freshwater systems, ranging from studies of aquatic organisms, physical and chemical works which relate to the biological environment, to ecological applications and frameworks for water management directives. Main topics: Ecology of freshwater systems ; biodiversity, taxonomy, distribution patterns in space and time, biology of animals and plants ; experimental and conceptual studies which integrate laboratory and/or field work on physiology, population dynamics, biogeochemistry and nutrient dynamics, management, mathematical modelling ; techniques for sampling and chemical analyses, ecological applications, procedures which provide frameworks for environmental legislation.