B. Rani-Borges, L. G. Queiroz, Karen de Souza Ferreira, Thaís Fabiane Gomes Martins, E. Vicente, R. A. Ando, Marcelo Pompêo
In recent years, there has been a growing interest in the impacts caused by the presence of microplastics (MP) in aquatic environments. The impacts of microalgae exposure to microplastics are still insufficiently investigated and further studies are needed to understand the possible outcomes. In addition, much of the literature has focused on the study of concentrations above those found naturally in the environment and in less toxic polymer matrices. Acrylonitrile-butadiene-styrene (ABS) plastics have a composition rich in additives and, so far, have been studied superficially. In the present study, two of the most commonly used green microalgae species in toxicity assays, Chlorella vulgaris and Raphidocelis subcapitata, were exposed to different concentrations of primary ABS-MP for a period of 6 days. Here, we observed physiological changes in cell growth and chlorophyll a content induced by the concentration and time of exposure to ABS-MP. The lowest concentration did not prove to be potentially toxic to cells, while the highest concentration was the most toxic. Primary consumers, such as microalgae, are essential for the proper functioning of entire ecosystems. Changes in these communities can lead to permanent damage to the communities of organisms at higher levels, so it is essential that their study be done carefully in the face of threats such as MP.
{"title":"Effects of ABS microplastics on microalgae Chlorella vulgaris and Raphidocelis subcapitata","authors":"B. Rani-Borges, L. G. Queiroz, Karen de Souza Ferreira, Thaís Fabiane Gomes Martins, E. Vicente, R. A. Ando, Marcelo Pompêo","doi":"10.1051/limn/2023006","DOIUrl":"https://doi.org/10.1051/limn/2023006","url":null,"abstract":"In recent years, there has been a growing interest in the impacts caused by the presence of microplastics (MP) in aquatic environments. The impacts of microalgae exposure to microplastics are still insufficiently investigated and further studies are needed to understand the possible outcomes. In addition, much of the literature has focused on the study of concentrations above those found naturally in the environment and in less toxic polymer matrices. Acrylonitrile-butadiene-styrene (ABS) plastics have a composition rich in additives and, so far, have been studied superficially. In the present study, two of the most commonly used green microalgae species in toxicity assays, Chlorella vulgaris and Raphidocelis subcapitata, were exposed to different concentrations of primary ABS-MP for a period of 6 days. Here, we observed physiological changes in cell growth and chlorophyll a content induced by the concentration and time of exposure to ABS-MP. The lowest concentration did not prove to be potentially toxic to cells, while the highest concentration was the most toxic. Primary consumers, such as microalgae, are essential for the proper functioning of entire ecosystems. Changes in these communities can lead to permanent damage to the communities of organisms at higher levels, so it is essential that their study be done carefully in the face of threats such as MP.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"94 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76562609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Betül Güroy, Derya Güroy, Serhan Mantoğlu, Onur Karadal, S. Oğuzkan
Spirulina (Arthrospira platensis Gomont, 1892) is grown in highly alkaline waters and is an important ingredient for many industries. However, analytical grade-based media in used commercial-scale production of Spirulina is quite expensive. Spirulina cultivation was studied by replacing the Schlösser medium with geothermal water in terms of its biomass, biochemical content, amino acid and mineral production. Spirulina was cultured in a semi-open raceway system with a 420 L culture volume for three weeks. Then, the geothermal water (GW) was replaced with 25% (GW25), 50% (GW50), 75% (GW75) and 100% (GW100) of the volume of the Schlösser culture medium (SM). The biomass concentration was determined to the higher in the GW50 (1.324 g/L) than in other groups (GW25, GW75, GW100 and SM), while the minimum yield was in GW100 (0.624 g/L) group. The highest protein content and phycocyanin purity ratio were found in the GW75 medium containing 116.6 times higher phosphate than the SM. The chlorophyll content of SM, GW25 and GW50 was higher than that of the GW100 (P < 0.05). The highest lipid content was determined in the GW100 (P < 0.05). Isoleucine, leucine and valine levels of the GW50 and GW75 were higher than those of the other groups (P < 0.05). The iron levels of the GW50 and GW75 groups, which have the highest nitrate content in the culture mediums, were significantly lower. The chlorophyll, phycocyanin, protein, dry biomass, mineral and amino acid in groups (GW25, GW50 and GW75) prepared by mixing Schlösser medium and geothermal water mix were better than geothermal water (GW100) and SM. As a result, it is concluded that it is possible to use partial geothermal water in Spirulina production under this study conditions.
{"title":"Geothermal water as a nutrient medium source on the biomass productivity and biochemical composition of Spirulina (Arthrospira platensis) under the greenhouse conditions","authors":"Betül Güroy, Derya Güroy, Serhan Mantoğlu, Onur Karadal, S. Oğuzkan","doi":"10.1051/limn/2023001","DOIUrl":"https://doi.org/10.1051/limn/2023001","url":null,"abstract":"Spirulina (Arthrospira platensis Gomont, 1892) is grown in highly alkaline waters and is an important ingredient for many industries. However, analytical grade-based media in used commercial-scale production of Spirulina is quite expensive. Spirulina cultivation was studied by replacing the Schlösser medium with geothermal water in terms of its biomass, biochemical content, amino acid and mineral production. Spirulina was cultured in a semi-open raceway system with a 420 L culture volume for three weeks. Then, the geothermal water (GW) was replaced with 25% (GW25), 50% (GW50), 75% (GW75) and 100% (GW100) of the volume of the Schlösser culture medium (SM). The biomass concentration was determined to the higher in the GW50 (1.324 g/L) than in other groups (GW25, GW75, GW100 and SM), while the minimum yield was in GW100 (0.624 g/L) group. The highest protein content and phycocyanin purity ratio were found in the GW75 medium containing 116.6 times higher phosphate than the SM. The chlorophyll content of SM, GW25 and GW50 was higher than that of the GW100 (P < 0.05). The highest lipid content was determined in the GW100 (P < 0.05). Isoleucine, leucine and valine levels of the GW50 and GW75 were higher than those of the other groups (P < 0.05). The iron levels of the GW50 and GW75 groups, which have the highest nitrate content in the culture mediums, were significantly lower. The chlorophyll, phycocyanin, protein, dry biomass, mineral and amino acid in groups (GW25, GW50 and GW75) prepared by mixing Schlösser medium and geothermal water mix were better than geothermal water (GW100) and SM. As a result, it is concluded that it is possible to use partial geothermal water in Spirulina production under this study conditions.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"578 1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76314715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To investigate the relationship between ostracod occurrence patterns, community assemblage, and abiotic factors, we sampled five sites on lake Karamurat (Bolu, Turkey) and two nearby rheocrene springs. Thirteen ostracod taxa (10 from the lake, three from the springs) were collected. Species exhibited clear habitat preferences, and lake and spring ostracods showed clear differences in their monthly and seasonal occurrences. Darwinula stevensoni and Cypria ophtalmica were the dominant species for the lake and Psychrodromus cf. fontinalis and P. olivaceus were only reported from the springs. Ostracod Watch Model illustrated that a rare species, Notodromas monacha, was only found in May to August from the lake while two species (D. stevensoni, C. ophtalmica) were encountered from all year around. Common species also exhibited relatively high levels of ecological tolerances to multiple environmental variables. Canonical correspondence analyses explained about 91% of correlation between species and environmental variables and indicated that four variables (water temperature, dissolved oxygen, pH and electrical conductivity) most strongly influenced species occurrences. Also, binary data of sample medians showed significant (P < 0.01) differences between ostracod assemblages from 13 lakes and reservoirs compared to Lake Karamurat. A significant correlation was detected between Lake Karamurat and two other lakes (Abant and Yeniçağa) located in the same region. The Pseudorichness Ratio (noncosmopolitan/cosmopolitan species) of the lake was very low (Pr = 0.25), indicating dominancy of cosmopolitan species over noncosmopolitans. Conservation efforts should be considered to addressed increasing anthropogenic impacts to Lake Karamurat.
{"title":"Ostracoda (Crustacea) and limnoecological characteristics of Lake Karamurat (Bolu, Turkey): Testing pseudorichness hypothesis","authors":"O. Külköylüoğlu","doi":"10.1051/limn/2022017","DOIUrl":"https://doi.org/10.1051/limn/2022017","url":null,"abstract":"To investigate the relationship between ostracod occurrence patterns, community assemblage, and abiotic factors, we sampled five sites on lake Karamurat (Bolu, Turkey) and two nearby rheocrene springs. Thirteen ostracod taxa (10 from the lake, three from the springs) were collected. Species exhibited clear habitat preferences, and lake and spring ostracods showed clear differences in their monthly and seasonal occurrences. Darwinula stevensoni and Cypria ophtalmica were the dominant species for the lake and Psychrodromus cf. fontinalis and P. olivaceus were only reported from the springs. Ostracod Watch Model illustrated that a rare species, Notodromas monacha, was only found in May to August from the lake while two species (D. stevensoni, C. ophtalmica) were encountered from all year around. Common species also exhibited relatively high levels of ecological tolerances to multiple environmental variables. Canonical correspondence analyses explained about 91% of correlation between species and environmental variables and indicated that four variables (water temperature, dissolved oxygen, pH and electrical conductivity) most strongly influenced species occurrences. Also, binary data of sample medians showed significant (P < 0.01) differences between ostracod assemblages from 13 lakes and reservoirs compared to Lake Karamurat. A significant correlation was detected between Lake Karamurat and two other lakes (Abant and Yeniçağa) located in the same region. The Pseudorichness Ratio (noncosmopolitan/cosmopolitan species) of the lake was very low (Pr = 0.25), indicating dominancy of cosmopolitan species over noncosmopolitans. Conservation efforts should be considered to addressed increasing anthropogenic impacts to Lake Karamurat.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"27 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78629429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"My first year as Editor-in-Chief of IJL","authors":"Stéphane Jacquet","doi":"10.1051/limn/2023003","DOIUrl":"https://doi.org/10.1051/limn/2023003","url":null,"abstract":"","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79766577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dujuan Dai, Yue Yang, Feihu Wang, Yang Zhang, Man Zhang, Yunni Gao, Xiao-Bing Gao, Jing Dong, Xuejun Li, Mengyang Chang
Interspecific interaction between submerged macrophytes and phytoplankton is of great significance in sustaining ecological balance and restoring eutrophic water regions. In consideration of the diversified algal density and macrophytes biomass, the present study selected two biomass levels of Egeria densa and gradient densities of Chlorella vulgaris for simulated cultivation experimentation. E. densa could significantly suppress the growth and affect the morphology of C. vulgaris. The allelopathically inhibitory growth of C. vulgaris was suggested to increase with increasing biomass of E. densa and decreased with the elevating density of C. vulgaris. Higher amount of colonies and bigger colony size of C. vulgaris were detected after the treatment with 5 g/L of E. densa together with C. vulgaris (OD680 = 0.2) and treatment with 10 g/L E. densa together with C. vulgaris (OD680 = 0.05). Elevated extracellular and intracellular polysaccharides were detected, which possibly contributed to the morphological changes of C. vulgaris induced by E. densa.
{"title":"Allelopathic effects of Egeria densa on the growth and morphology of Chlorella vulgaris","authors":"Dujuan Dai, Yue Yang, Feihu Wang, Yang Zhang, Man Zhang, Yunni Gao, Xiao-Bing Gao, Jing Dong, Xuejun Li, Mengyang Chang","doi":"10.1051/limn/2023004","DOIUrl":"https://doi.org/10.1051/limn/2023004","url":null,"abstract":"Interspecific interaction between submerged macrophytes and phytoplankton is of great significance in sustaining ecological balance and restoring eutrophic water regions. In consideration of the diversified algal density and macrophytes biomass, the present study selected two biomass levels of Egeria densa and gradient densities of Chlorella vulgaris for simulated cultivation experimentation. E. densa could significantly suppress the growth and affect the morphology of C. vulgaris. The allelopathically inhibitory growth of C. vulgaris was suggested to increase with increasing biomass of E. densa and decreased with the elevating density of C. vulgaris. Higher amount of colonies and bigger colony size of C. vulgaris were detected after the treatment with 5 g/L of E. densa together with C. vulgaris (OD680 = 0.2) and treatment with 10 g/L E. densa together with C. vulgaris (OD680 = 0.05). Elevated extracellular and intracellular polysaccharides were detected, which possibly contributed to the morphological changes of C. vulgaris induced by E. densa.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"54 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80718225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Płóciennik, Violeta Berlajolli, D. Dmitrović, B. Gligorović, V. Pešić, P. Gadawski
Chironomidae are known to occur in small, even astatic water bodies like seepages, rheocrens, pools and wells. The Balkan Peninsula reveals a high variability of small water bodies, with springs (rheocrens, limnocrens and helocrens) being the most widely distributed. In this review, we give a brief presentation of the Chironomidae communities in valley and mountain small water bodies, and in Lake Skadar (Shkodra) spring system. River valleys offer a large variety of small freshwater habitats. Their presence strongly increases of midge (Chironomidae) diversity, providing a habitat for the cold-stenotherms and semiterrestrial taxa that do not occur in rivers and lakes. The species richness in small water bodies strongly depends on their hydrological conditions (i.e. perennial vs. astatic water bodies), size and microhabitat complexity. Mountain spring communities depend on precipitation and exhibit altitudinal zonation. The higher mountain zones (1400–1500 m a.s.l.) have the most diverse midge assemblages, due to their stable perennial hydrological conditions. Human activity may alter species composition in riparian springs, favouring taxa that are not typical to the local fauna. By studying these small aquatic habitats, the significance of their Chironomidae fauna is being recognised, thereby filling a gap in the knowledge of freshwater insects biodiversity in the Balkan region.
{"title":"The Chironomidae (Diptera) diversity in the Balkan Peninsula spring systems and other small water bodies","authors":"M. Płóciennik, Violeta Berlajolli, D. Dmitrović, B. Gligorović, V. Pešić, P. Gadawski","doi":"10.1051/limn/2023005","DOIUrl":"https://doi.org/10.1051/limn/2023005","url":null,"abstract":"Chironomidae are known to occur in small, even astatic water bodies like seepages, rheocrens, pools and wells. The Balkan Peninsula reveals a high variability of small water bodies, with springs (rheocrens, limnocrens and helocrens) being the most widely distributed. In this review, we give a brief presentation of the Chironomidae communities in valley and mountain small water bodies, and in Lake Skadar (Shkodra) spring system. River valleys offer a large variety of small freshwater habitats. Their presence strongly increases of midge (Chironomidae) diversity, providing a habitat for the cold-stenotherms and semiterrestrial taxa that do not occur in rivers and lakes. The species richness in small water bodies strongly depends on their hydrological conditions (i.e. perennial vs. astatic water bodies), size and microhabitat complexity. Mountain spring communities depend on precipitation and exhibit altitudinal zonation. The higher mountain zones (1400–1500 m a.s.l.) have the most diverse midge assemblages, due to their stable perennial hydrological conditions. Human activity may alter species composition in riparian springs, favouring taxa that are not typical to the local fauna. By studying these small aquatic habitats, the significance of their Chironomidae fauna is being recognised, thereby filling a gap in the knowledge of freshwater insects biodiversity in the Balkan region.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"11 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78811453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Maraşlıoğlu, Tolga Coşkun, T. Çetin, Nazmi Kağnıcıoğlu, F. Ekmekçi, M. Şahin
The objective of this research was to assess the ecological status of lentic systems in the Eastern Mediterranean River basin (Türkiye) using some biotic (Q index, PT-BV, MedPTI, TDIL, and PTI) and abiotic (WQI, WQImin-nw, TSI, TLI, and Kna) indices. Phytoplankton species such as Peridinium cinctum, Ceratium hirundinella, and Gyrosigma balticum were the species that contributed the most to the algal biovolume of lake systems with different ecological statuses in the basin. According to the results, it was seen that CCA coordination, which analyzed the relationship structures between dominant phytoplankton taxa and environmental water quality parameters, explained the variation sufficiently. As pointed out in the CCA analysis, conductivity and ammonium were the main environmental parameters influencing algal assemblages at sampling sites in the basin (p < 0.01). Strong correlations were observed between TSI and TLI (correlation coefficient: 0.99), and TDIL showed significant correlation only with the Q index (p < 0.01). TSI, TLI and WQI, abiotic indices, indicated significant correlations with most environmental parameters (p < 0.01), while PTI, a biotic index, had weak correlations with most environmental parameters (p > 0.05). Among the indices used in this study, it seems that diatom-based TDIL and physicochemical-based WQI appear to be the most suitable indices for assessing the ecological status of lentic systems in the Mediterranean region. Accordingly, it can be deduced that coupling biotic and abiotic indices is more accurate in determining the water quality of lentic systems.
{"title":"Use of different indices to assess the ecological status of lake systems in the eastern mediterranean river basin","authors":"F. Maraşlıoğlu, Tolga Coşkun, T. Çetin, Nazmi Kağnıcıoğlu, F. Ekmekçi, M. Şahin","doi":"10.1051/limn/2023007","DOIUrl":"https://doi.org/10.1051/limn/2023007","url":null,"abstract":"The objective of this research was to assess the ecological status of lentic systems in the Eastern Mediterranean River basin (Türkiye) using some biotic (Q index, PT-BV, MedPTI, TDIL, and PTI) and abiotic (WQI, WQImin-nw, TSI, TLI, and Kna) indices. Phytoplankton species such as Peridinium cinctum, Ceratium hirundinella, and Gyrosigma balticum were the species that contributed the most to the algal biovolume of lake systems with different ecological statuses in the basin. According to the results, it was seen that CCA coordination, which analyzed the relationship structures between dominant phytoplankton taxa and environmental water quality parameters, explained the variation sufficiently. As pointed out in the CCA analysis, conductivity and ammonium were the main environmental parameters influencing algal assemblages at sampling sites in the basin (p < 0.01). Strong correlations were observed between TSI and TLI (correlation coefficient: 0.99), and TDIL showed significant correlation only with the Q index (p < 0.01). TSI, TLI and WQI, abiotic indices, indicated significant correlations with most environmental parameters (p < 0.01), while PTI, a biotic index, had weak correlations with most environmental parameters (p > 0.05). Among the indices used in this study, it seems that diatom-based TDIL and physicochemical-based WQI appear to be the most suitable indices for assessing the ecological status of lentic systems in the Mediterranean region. Accordingly, it can be deduced that coupling biotic and abiotic indices is more accurate in determining the water quality of lentic systems.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"26 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89045233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DNA metabarcoding can be a promising alternative to microscopy for analysing phytoplankton, a key ecological indicator for freshwater ecosystems. The aim of this study was to evaluate the performance of different barcodes and associated primer pairs to assess microalgal diversity with DNA metabarcoding using a single barcode targeting all microalgae. We investigated barcodes in 16S and 23S rRNA genes, encoding for prokaryotic ribosomal sub-units, that are present in Cyanobacteria as well as in chloroplasts. In silico PCR tests were carried out on eight 16S and five 23S primer pairs using the Phytool reference library. Two and three pairs were selected for 16S and 23S, respectively, to perform an in vitro metabarcoding test based on a mock community made of DNA extracts of 10 microalgae strains. The 23S pairs enabled to detect all species, whereas 16S ones failed in the detection of some of them. One pair was selected for each genetic marker, based on its efficiency and specificity towards microalgae ( e.g. not heterotrophic bacteria). Another mock community covering a larger diversity (18 microalgae strains) was used to test the efficiency of the selected pairs and their ability to estimate relative abundances. The 23S pair performed better than the 16S one for detecting target species with also more accuracy to assess their relative abundances. We conclude that the 23S primer pair ECLA23S_F1/ECLA23S_R1 appears as a good candidate to decipher freshwater phytoplankton communities. As a next step, it will be necessary to confirm these results on a large diversity of natural communities.
{"title":"Which barcode to decipher freshwater microalgal assemblages? Tests on mock communities","authors":"Alexis Canino, Clarisse Lemonnier, Benjamin Alric, Agnès Bouchez, Isabelle Domaizon, Christophe Laplace-Treyture, Frédéric Rimet","doi":"10.1051/limn/2023008","DOIUrl":"https://doi.org/10.1051/limn/2023008","url":null,"abstract":"DNA metabarcoding can be a promising alternative to microscopy for analysing phytoplankton, a key ecological indicator for freshwater ecosystems. The aim of this study was to evaluate the performance of different barcodes and associated primer pairs to assess microalgal diversity with DNA metabarcoding using a single barcode targeting all microalgae. We investigated barcodes in 16S and 23S rRNA genes, encoding for prokaryotic ribosomal sub-units, that are present in Cyanobacteria as well as in chloroplasts. In silico PCR tests were carried out on eight 16S and five 23S primer pairs using the Phytool reference library. Two and three pairs were selected for 16S and 23S, respectively, to perform an in vitro metabarcoding test based on a mock community made of DNA extracts of 10 microalgae strains. The 23S pairs enabled to detect all species, whereas 16S ones failed in the detection of some of them. One pair was selected for each genetic marker, based on its efficiency and specificity towards microalgae ( e.g. not heterotrophic bacteria). Another mock community covering a larger diversity (18 microalgae strains) was used to test the efficiency of the selected pairs and their ability to estimate relative abundances. The 23S pair performed better than the 16S one for detecting target species with also more accuracy to assess their relative abundances. We conclude that the 23S primer pair ECLA23S_F1/ECLA23S_R1 appears as a good candidate to decipher freshwater phytoplankton communities. As a next step, it will be necessary to confirm these results on a large diversity of natural communities.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135549667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We evaluated the impact of altitude on the biodiversity and water quality of 13 springs located from 82 to 1,189 m a.s.l. on Mount Cameroon. The springs were of good chemical and ecological quality whatever the altitude. The analysis of the physicochemical variables showed low temperature levels (19.50 ± 2.09 °C), high turbidity (13.0 ± 7.17 FTU), and an acceptable mineralization level (324.95 ± 260.0 μS/cm), with high amounts of phosphate nutrients (0.83 ± 0.47 mg/L). We observed a strong seasonal effect, with a decrease in temperature and nutrient content during the dry season whereas pH and dissolved oxygen increased. A total of 10,265 organisms, distributed into 56 families, were collected. They mostly included insects (47.8%), closely followed by Arachnida (34.8%). Only two stygobite taxa were recorded, namely Darwinulidae and Stenasellidae. Total diversity slightly decreased with altitude, especially during the dry season. Despite lower temperature and more oxygen at higher altitudes, diversity, including EPT did not increase. Therefore, African fauna are less sensitive to rising temperature than the faunas of other areas of the world. This result may be explained either by the fact that African species are better adapted to warm, low-oxygen waters than species from other parts of the world, or by the absence of refuges in Mount Cameroon that are home to temperature-sensitive species. Consequently, the impact of climate change on aquatic macroinvertebrates in tropical Africa could be reduced.
{"title":"Impact of altitude on spring macroinvertebrates and water quality in South West region of Cameroon","authors":"Sylvie Belengfe Chinche, Christophe Piscart, Pascale Mbanga Medjo, Ernest Koji, Raoul Polycarpe Tuekam Kayo, Serge Hubert Zebaze Togouet","doi":"10.1051/limn/2023010","DOIUrl":"https://doi.org/10.1051/limn/2023010","url":null,"abstract":"We evaluated the impact of altitude on the biodiversity and water quality of 13 springs located from 82 to 1,189 m a.s.l. on Mount Cameroon. The springs were of good chemical and ecological quality whatever the altitude. The analysis of the physicochemical variables showed low temperature levels (19.50 ± 2.09 °C), high turbidity (13.0 ± 7.17 FTU), and an acceptable mineralization level (324.95 ± 260.0 μS/cm), with high amounts of phosphate nutrients (0.83 ± 0.47 mg/L). We observed a strong seasonal effect, with a decrease in temperature and nutrient content during the dry season whereas pH and dissolved oxygen increased. A total of 10,265 organisms, distributed into 56 families, were collected. They mostly included insects (47.8%), closely followed by Arachnida (34.8%). Only two stygobite taxa were recorded, namely Darwinulidae and Stenasellidae. Total diversity slightly decreased with altitude, especially during the dry season. Despite lower temperature and more oxygen at higher altitudes, diversity, including EPT did not increase. Therefore, African fauna are less sensitive to rising temperature than the faunas of other areas of the world. This result may be explained either by the fact that African species are better adapted to warm, low-oxygen waters than species from other parts of the world, or by the absence of refuges in Mount Cameroon that are home to temperature-sensitive species. Consequently, the impact of climate change on aquatic macroinvertebrates in tropical Africa could be reduced.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135007514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Many zooplankton organisms facing harsh environmental conditions producing resting eggs. Resting eggs accumulate in the sediment and create a resting egg bank. Knowledge on the egg bank structure, morphology and morphometry of the eggs as well as the effect of cues on hatching, contributes to the understanding zooplankton dynamics in lakes. Here we described the composition and structure of zooplankton egg bank from a shallow tropical lake and analyzed the effect of some biotic factors on hatching rates. In order to describe the structure and composition of the resting egg bank, we determined the richness, density, morphology and morphometry of the resting eggs isolated by the sugar flotation method. Diapausing eggs were measured and their external features studied by microphotography under optical microscope and SEM. To analyze the effect of biotic factors on hatching rates, we exposed the resting structures and the entire sediment to three biotic factors: a chlorophyte (Scenedesmus acutus), a cyanobacteria (Microcystis sp.) and a predatory rotifer (Asplanchna girodi). A total of 25 zooplankton species hatched from the sediments. Our results show that the medium density of the healthy-looking diapausing eggs was of 7.6 ± 2 diapausing eggs · cm−3 and that rotifers are the predominant group in the egg bank. Medium conditioned with the chlorophyte and the cyanobacteria resulted in a higher hatching rate. The ornamentations of rotifer diapausing eggs present different features, such as wrinkles and spines, which can help to taxonomic identification without the need of promoting hatching.
{"title":"Zooplankton egg bank: characterization and effect of biotic factors on hatching","authors":"Jair García-Zamorano, J. Jiménez-Contreras","doi":"10.1051/limn/2023002","DOIUrl":"https://doi.org/10.1051/limn/2023002","url":null,"abstract":"Many zooplankton organisms facing harsh environmental conditions producing resting eggs. Resting eggs accumulate in the sediment and create a resting egg bank. Knowledge on the egg bank structure, morphology and morphometry of the eggs as well as the effect of cues on hatching, contributes to the understanding zooplankton dynamics in lakes. Here we described the composition and structure of zooplankton egg bank from a shallow tropical lake and analyzed the effect of some biotic factors on hatching rates. In order to describe the structure and composition of the resting egg bank, we determined the richness, density, morphology and morphometry of the resting eggs isolated by the sugar flotation method. Diapausing eggs were measured and their external features studied by microphotography under optical microscope and SEM. To analyze the effect of biotic factors on hatching rates, we exposed the resting structures and the entire sediment to three biotic factors: a chlorophyte (Scenedesmus acutus), a cyanobacteria (Microcystis sp.) and a predatory rotifer (Asplanchna girodi). A total of 25 zooplankton species hatched from the sediments. Our results show that the medium density of the healthy-looking diapausing eggs was of 7.6 ± 2 diapausing eggs · cm−3 and that rotifers are the predominant group in the egg bank. Medium conditioned with the chlorophyte and the cyanobacteria resulted in a higher hatching rate. The ornamentations of rotifer diapausing eggs present different features, such as wrinkles and spines, which can help to taxonomic identification without the need of promoting hatching.","PeriodicalId":7903,"journal":{"name":"Annales De Limnologie-international Journal of Limnology","volume":"50 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72871841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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