Design an Enzyme-Less Sensitive Sensor for Cholesterol Detection Based on Poly Brucine/Polyvinylpyrrolidone Film

Abstract

In the current study, the main focus is designing a new enzyme-less modified electrode based on the electro deposition of Poly Brucine/Polyvinylpyrrolidone on pencil graphite electrode and its utilizing for cholesterol quantification. The electrochemical responses of the proposed sensor were recorded using cyclic and square wave voltammetry techniques for detection of cholesterol at optimized pH =2. The morphology investigations of the modified surfaces were performed by scanning electron microscopy and the obtained images verified Nano formations in the structure of the sensor. The proposed electrochemical sensor revealed a good electro catalytic response to cholesterol concerning the parameters α = 0.83, log Ks = 3.11 and Γ = 8.55 × 10T8. Besides, the calibration plot was acquired with a linear portion in the concentration ranges of 25 to 95 nmol L−1 (nM) and the detection limit (LOD = 8.28 nM). The designed sensor demonstrated the reproducible and repeat-able outputs, the high sensitivity and stability in analyzing cholesterol as well as recoveries in blood serum samples without any side serious interferences.