Sem-sterile culture of Gracilaria salicornia seaweed (parental) on various media

PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019 Pub Date : 2019-12-23 DOI:10.1063/1.5141331

S. Y. Lumbessy

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引用次数: 1

Abstract

Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.

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海葛菜(亲本)在不同培养基上的半无菌培养

半无菌培养是获得无菌海藻外植体(无菌外植体)的初始维持阶段。本研究的目的是研究在半无菌培养条件下，使用不同的培养基对海藻草(亲本)增重和成活率的影响。这项研究是在西努沙登加拉Sekotong龙目岛海洋水产养殖中心组织培养实验室进行的。该研究采用Provasoli富集海水(PES)、水培海水(hydroponic)和Steril海水(SSW)三种不同的培养基处理作为对照。结果表明，水培培养基对海角藻的平均体重和存活率最高，分别为48.76 g和99.75%。结果与SSW培养基差异不显著，但与PES培养基差异显著。半无菌培养是获得无菌海藻外植体(无菌外植体)的初始维持阶段。本研究的目的是研究在半无菌培养条件下，使用不同的培养基对海藻草(亲本)增重和成活率的影响。这项研究是在西努沙登加拉Sekotong龙目岛海洋水产养殖中心组织培养实验室进行的。该研究采用Provasoli富集海水(PES)、水培海水(hydroponic)和Steril海水(SSW)三种不同的培养基处理作为对照。结果表明，水培培养基对海角藻的平均体重和存活率最高，分别为48.76 g和99.75%。结果与SSW培养基差异不显著，但与PES培养基差异显著。

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PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019

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