Phytochemical Analysis and Efficacy Of Rosemary (Rosmarinus Officinalis) and Mint (Mentha Spicata) Extracts Against Fall Armyworm (Spodoptera Frugiperda) on Baby Corn (Zea Mays)

Redempta S. Kalinda, N. B. Rioba
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Abstract

Abstract Fall armyworm (Spodoptera frugiperda, J.E. Smith) is a pest with devasting effects on maize. A laboratory biassay was conducted to analyse the phytochemicals and determine the efficacy of M. spicata and R. officinalis extracts on FAW. Treatments were laid out in a Completely Randomized Design (CRD) with 3 replications. The factors included solvent [Methanol (Me), dichloromethane (DCM), distilled water (Di)] and the plant species (M. spicata and R. officinalis). Coragen SC 200 (Co) and Distilled water (Di) were the positive and negative controls, respectively. FAW rearing, plant extract preparation and phytochemical screening were done using standard procedure. Data collection and analysis was done using standard procedures. The extract yield was highest for R. officinalis regardless of the solvent used. Me-R. officinalis and Di-M. spicata extracts yielded the highest. Saponins, glycosides, alkaloid, flavonoids and tannins. Flavonoid contents were 7.9036 mg/mL and 6.0073 ± 0.6117 mg/mL in methanolic extract of M. spicata and R. officinalis, respectively. M. spicatha and R. officinalis extracts caused 100% mortality to 3rd instar larvae. Based on the findings, both M. spicata and R. officinalis have several secondary metabolites that confer insecticidal activity of the plants against FAW, hence should be evaluated under field conditions.
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迷迭香(Rosmarinus Officinalis)和薄荷(Mentha Spicata)提取物对幼玉米(Zea Mays)上秋粘虫(Spodoptera Frugiperda)的植物化学分析及防治效果
摘要秋粘虫(Spodoptera frugiperda, J.E. Smith)是一种危害玉米的害虫。采用室内双组份法,分析了spicata和officinalis提取物的植物化学成分,并确定了其对FAW的作用。处理采用完全随机设计(CRD), 3个重复。影响因子包括溶剂[甲醇(Me)、二氯甲烷(DCM)、蒸馏水(Di)]和植物种类(spicata和R. officinalis)。Coragen sc200 (Co)和蒸馏水(Di)分别为阳性对照和阴性对照。采用标准程序进行FAW饲养、植物提取物制备和植物化学筛选。使用标准程序进行数据收集和分析。无论采用何种溶剂,马蹄草的提取率均最高。r。officinalis和Di-M。Spicata提取物提取率最高。皂苷、糖苷、生物碱、类黄酮和单宁。黄酮含量分别为7.9036 mg/mL和6.0073±0.6117 mg/mL。三龄幼虫的死亡率为100%;基于这些发现,spicata和R. officinalis都有几种次生代谢物,这些代谢物赋予了植物对FAW的杀虫活性,因此应该在田间条件下进行评估。
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