Determination of Microcystis aeruginosa Concentration Using Two Discrete Wavelengths

Collin Prebenda, B. Fernandes, Thomas Griffin, Jonathan A. Markowitz, Keagan Carson, Yi Zheng
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Abstract

Harmful algal blooms (HABs), specifically Microcystis aeruginosa (MA), present a serious global problem to bodies of water. HABs are the rapid growth of toxic algae species in a waterway. These algae species are known to cause irritation, nausea, and vomiting in humans, and even more severe side effects in smaller organisms. Climate change and human development have caused these harmful blooms to become more prevalent in recent years. Current commercial and academic algae detection methods were researched and found to be highly restrictive or expensive. This creates the need for a monitoring device that fills this niche, which the team attempted to do. Regarding the detection of MA, the peak spectral absorbances were determined to be at wavelengths of 430 nm and 680 nm. The handheld harmful algae monitoring device directs these specific wavelengths of light matching the peak absorptions of MA through a sample. The relative intensity of light after passing through the sample is measured and used to determine the presence and concentration of MA. This detection method is low cost, is portable, and will provide efficient and precise results with the hope of enabling a variety of users on a large scale. With proper calibration and more research, the handheld harmful algae monitoring device has the potential of being highly accurate and capable of testing nonpure samples.
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双离散波长法测定铜绿微囊藻浓度
有害藻华(HABs),特别是铜绿微囊藻(Microcystis aeruginosa, MA),对水体造成了严重的全球性问题。有害藻华是指水道中有毒藻类的快速生长。众所周知,这些藻类会对人类造成刺激、恶心和呕吐,对较小的生物甚至会产生更严重的副作用。近年来,气候变化和人类发展导致这些有害的水华变得更加普遍。目前的商业和学术藻类检测方法进行了研究,发现限制很大或价格昂贵。这就需要一种监测设备来填补这一空白,这也是该团队试图做的。对于MA的检测,确定了在430 nm和680 nm波长处的光谱吸光度峰值。手持式有害藻类监测设备引导这些特定波长的光与MA的峰值吸收相匹配,通过样品。光通过样品后的相对强度被测量,并用于确定MA的存在和浓度。这种检测方法成本低,便携,将提供高效、精确的结果,有望使各种用户大规模使用。通过适当的校准和更多的研究,手持式有害藻类监测设备具有高度准确性和测试非纯样品的能力。
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