{"title":"INVESTIGATION OF ELECTROCHEMICAL PROPERTIES OF UA AT AL2O3NP/CPE AND DEVELOPMENT OF VOLTAMMETRIC METHOD FOR ITS DETERMINATION IN SERUM","authors":"Rüveyda Üver, F. Öztürk","doi":"10.15671/hjbc.1225829","DOIUrl":null,"url":null,"abstract":"The electrochemical properties of uric acid (UA) were determined by cyclic voltammetry (CV) and square wave voltammetry (SWV) at a carbon paste electrode modified with aluminum oxide nanoparticles (Al2O3NP/CPE) in 0.05 M pH 5.0 phosphate buffer. The adsorption properties of the molecule on Al2O3NP/CPE were investigated and the number of electrons transferred in the electrode reaction was calculated. A new voltammetric method for the determination of UA in human serum was also proposed. The linear operating range and limit of detection (LOD) of the method were found to be 0.1µM-230 µM and 0.1µM, respectively. The developed method for UA in serum showed high reliability, reproducibility, accuracy and precision.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"288 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hacettepe Journal of Biology and Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15671/hjbc.1225829","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The electrochemical properties of uric acid (UA) were determined by cyclic voltammetry (CV) and square wave voltammetry (SWV) at a carbon paste electrode modified with aluminum oxide nanoparticles (Al2O3NP/CPE) in 0.05 M pH 5.0 phosphate buffer. The adsorption properties of the molecule on Al2O3NP/CPE were investigated and the number of electrons transferred in the electrode reaction was calculated. A new voltammetric method for the determination of UA in human serum was also proposed. The linear operating range and limit of detection (LOD) of the method were found to be 0.1µM-230 µM and 0.1µM, respectively. The developed method for UA in serum showed high reliability, reproducibility, accuracy and precision.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
