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COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS 生物聚合物包覆电纺丝支架的神经胶质保护作用比较
Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1245678
Ece BATTALOĞLU, Zehra Gül MORÇİMEN, Aylin ŞENDEMİR
Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone (PCL), hyaluronic acid (HA) coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent (IF) staining was performed with glial fibrillary acidic protein (GFAP) antibody to determine the level of gliosis. It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.
神经胶质瘤是一种在各种神经系统疾病中起负面作用的疾病。本研究旨在获得并比较不同生物聚合物包被的电纺丝纤维支架的神经胶质保护作用。首先,用LPS和IFN-γ诱导U-87 MG细胞形成体外胶质瘤模型。将优化后的模型应用于生长在聚己内酯(PCL)、透明质酸(HA)包被、明胶包被和胶原包被PCL支架上的U-87 MG细胞。采用胶质原纤维酸性蛋白(GFAP)抗体进行免疫荧光(IF)染色,检测胶质瘤的发生程度。结果发现,5µg/mL LPS诱导的胶质细胞形成和透明质酸包被的PCL支架对胶质细胞形成具有保护作用。
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引用次数: 0
Assessment of Staphylococcal toxins acting as superantigens in different nasal specimens in the etiology of chronic rhinosinusitis 慢性鼻窦炎病因学中不同鼻标本中葡萄球菌毒素作为超级抗原的评估
Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1302331
Gülden VURAL, Alper CEYLAN, M. Nedim SULTAN
The mechanism of development of chronic rhinosinusitis(CRS) is not fully known. However, bacteria are thought to play an important role in this clinic. It has been suggested that toxins with superantigen(SAgs) properties produced by one of these bacteria, Staphylococcus aureus(S.aureus), play a role in the development of inflammation associated with sinusitis. In this study, S.aureus was isolated by taking endoscopic sinus biopsy samples and nasal swab samples from patients with CRS and the control group. It was aimed to examine the frequency of S.aureus presence in the samples taken, the presence of toxin genes showing superantigen quality in these isolated bacteria, and to evaluate the roles of these parameters in the development of CRS. More S.aureus was isolated in the samples taken from patients with CRS than in the control group. The isolated S.aureus samples were analysed by real-time PCR method. The presence of enterotoxin A, B, C and D genes in the S.aureus samples isolated from the patient group were found at the rates of 54%, 32%, 16% and 16%, respectively, while these rates were 46%, 24%, 14% and 14% in the control group. The Toxic Shock Syndrome Toxin-1(TSST-1) gene was detected in 20% of the samples isolated from the patient and 46% in the control group bacteria. The fact that S.aureus was isolated in 20% of the patients shows that this bacterium is not necessary for CRS. The frequency of superantigen toxin genes in S.aureus isolates shows that these toxins are not necessary for the development of the disease.
慢性鼻窦炎(CRS)的发病机制尚不完全清楚。然而,细菌被认为在这种临床中起着重要作用。有研究表明,这些细菌中的一种,金黄色葡萄球菌(S.aureus)产生的具有超抗原(sag)特性的毒素在鼻窦炎相关炎症的发展中起作用。本研究通过对CRS患者和对照组的鼻内镜活检样本和鼻拭子样本分离金黄色葡萄球菌。目的是检测金黄色葡萄球菌在所取样品中的存在频率,这些分离细菌中显示超抗原质量的毒素基因的存在,并评估这些参数在CRS发展中的作用。与对照组相比,从CRS患者的样本中分离出更多的金黄色葡萄球菌。采用实时荧光定量PCR法对分离的金黄色葡萄球菌样品进行分析。患者组金黄色葡萄球菌样品中肠毒素A、B、C和D基因的检出率分别为54%、32%、16%和16%,而对照组的检出率分别为46%、24%、14%和14%。中毒性休克综合征毒素-1(TSST-1)基因在患者分离的样品中检测到20%,在对照组细菌中检测到46%。金黄色葡萄球菌在20%的患者中被分离的事实表明,这种细菌不是CRS所必需的。金黄色葡萄球菌分离物中超抗原毒素基因的频率表明,这些毒素对疾病的发展不是必需的。
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引用次数: 0
THE SHORT-TERM EFFECTS OF WHEAT STRAW CELLULOSE ON SOIL CARBON MINERALIZATION 麦秸纤维素对土壤碳矿化的短期影响
Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1270451
Bahar MERYEMOĞLU, Nacide KIZILDAĞ ÖZDAL
Cellulosic wastes constitute the majority of agricultural fields. The purpose of this study was to utilize these cellulosic wastes such as wheat straw and wheat straw cellulose on soil carbon mineralization in a sandy loam soils. Two different doses (100 and 1000 mg) of wheat straw cellulose were used to determine the carbon mineralization using CO2 respiration method. The cumulative carbon mineralization was found to be highest at the minumum doses of wheat straw cellulose with nitrogen (W-CL-N, 19.65 mg) and the lowest at the maximum doses of wheat straw (W-Straw, 14.32 mg). The results showed that the application of wheat straw cellulose at minumum doses resulted in higher carbon mineralization rate. The maximum carbon mineralization rate was observed in soil with minimum wheat straw cellulose and nitrogen source were added (1.41%). Whereas, the minimum carbon mineralization rate was determined in the soil mixed maximum wheat straw (1.03 %). The soil mixed maximum wheat straw was determined the lowest carbon mineralization rate due to its complex structure. The use of nitrogen source and organic matter with cellulose have positive effect on soil carbon mineralization. It might be said that these results describe an effective way for disposal of organic wastes.
大部分农田都是纤维素废料。本研究的目的是利用麦秸和麦秸纤维素等纤维素废弃物对砂壤土土壤碳矿化的影响。采用CO2呼吸法测定两种不同剂量(100和1000 mg)麦秸纤维素的碳矿化。小麦秸秆纤维素加氮最低剂量(W-CL-N, 19.65 mg)累积碳矿化最高,最大剂量(W-Straw, 14.32 mg)最低。结果表明,施用最低剂量的麦秸纤维素,碳矿化率较高。小麦秸秆纤维素和氮源添加量最少时,土壤碳矿化率最高(1.41%)。最大麦秸混合土壤的碳矿化率最低,为1.03%。混合最多麦秸的土壤因其结构复杂,碳矿化率最低。氮源和有机质配合纤维素的利用对土壤碳矿化有积极作用。可以说,这些结果描述了一种处理有机废物的有效途径。
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引用次数: 0
E. coli O157:H7 Detection Using Surface Plasmon Resonance Based Biosensor 基于表面等离子体共振的生物传感器检测大肠杆菌O157:H7
Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1271685
Esma ESER, Okan Öner EKİZ, H. İbrahim EKİZ
The detection of foodborne pathogenic bacteria remains a significant challenge, and the need for fast and sensitive detection methods is becoming increasingly important. Escherichia coli is a prevalent bacteria associated with foodborne illness, and this study aimed to evaluate the ability of a surface plasmon resonance (SPR) based biosensor to detect E. coli O157:H7 at low levels in pure culture and artificially contaminated bay leaves (Laurus nobilis) using different injection methods. To develop a biological sensing surface, the sensor surface was functionalized with 3-aminopropyltriethoxysilane (APTES), and polyclonal antibodies were immobilized on the surface for bacteria detection. Bacterial attachment to the antibodies resulted in a change in resonance angle. The biosensor was able to discriminate between cellular concentrations of 103 to 107 CFU/mL and showed potential in detecting different pathogens in various food samples. Before the SPR detection, the sample preparation step was optimized to ensure complex food matrices were suitable for SPR analysis. The results suggest that the SPR based biosensor is a promising tool for the rapid detection of foodborne pathogens in complex food matrices.
食源性致病菌的检测仍然是一项重大挑战,对快速、灵敏的检测方法的需求正变得越来越重要。大肠杆菌是一种与食源性疾病相关的常见细菌,本研究旨在评价基于表面等离子体共振(SPR)的生物传感器在不同注射方法的纯培养和人工污染月桂叶(月桂叶)中检测低水平大肠杆菌O157:H7的能力。利用3-氨基丙基三乙氧基硅烷(APTES)对传感器表面进行功能化,并将多克隆抗体固定在传感器表面进行细菌检测。细菌附着在抗体上导致共振角的改变。该生物传感器能够区分103至107 CFU/mL的细胞浓度,并显示出检测各种食品样品中不同病原体的潜力。在SPR检测前,对样品制备步骤进行优化,以确保复杂的食品基质适合SPR分析。结果表明,基于SPR的生物传感器是快速检测复杂食品基质中食源性病原体的一种有前景的工具。
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引用次数: 0
Content Analysis of Locally Marketed Energy Drinks: Turkish Market 土耳其市场本地销售的能量饮料含量分析
Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1279376
Zafer PEKER, Dursun Ali KÖSE
In the Turkish Food Codex Communique on Energy Drinks, energy drinks are defined as "flavored non-alcoholic beverage containing caffeine, containing taurine, glucuronolactone, inositol, carbohydrates, amino acids, vitamins, minerals, and other food and components". The amount of caffeine in the composition of the energy drink cannot be more than 150 mg/L, inositol 100 mg/L, glucuronolactone 20 mg/L, and taurine 800 mg/L. It has been reported that the consumption of beverages containing caffeine, taurine, and glucuronolactone in healthy young adults increases arterial blood pressure and platelet aggregation and decreases endothelial function. For healthy adults, consuming more than 400 mg of caffeine daily and consuming more than 500 ml of energy drinks in energy drinks, as well as consuming energy drinks with or together with alcohol, is risky and not recommended. Energy drinks are risky for children, people under the age of 18, the elderly, diabetics, those with high blood pressure, pregnant and lactating women, those with metabolic diseases, kidney failure, and people who are sensitive to caffeine. The use of energy drinks has been increasing rapidly in the world and in our country in recent years. Usually, energy drinks are used by young people, athletes, and people who live actively. Energy drinks are also preferred as a pleasure or to increase mental, physical, and cognitive performance. The aim of our study is to analyze the components of the content and the effectiveness of the components by making a qualitative analysis with the GC-MS (Gas Chromatography-Mass Analysis) device of many energy drinks used in the market. In addition, it is the evaluation of the energy drinks available in the market by examining the effects of the determined substances and their amounts on human health.
在土耳其食品法典关于能量饮料的公报中,能量饮料被定义为“含有咖啡因、牛磺酸、葡萄糖醛酸内酯、肌醇、碳水化合物、氨基酸、维生素、矿物质和其他食物和成分的调味非酒精饮料”。能量饮料中咖啡因的含量不能超过150毫克/升,肌醇不能超过100毫克/升,葡萄糖醛酸内酯不能超过20毫克/升,牛磺酸不能超过800毫克/升。据报道,健康年轻人饮用含有咖啡因、牛磺酸和葡萄糖醛酸内酯的饮料会增加动脉血压和血小板聚集,降低内皮功能。对于健康的成年人来说,每天摄入超过400毫克的咖啡因,在能量饮料中摄入超过500毫升的能量饮料,以及与酒精一起饮用或一起饮用能量饮料,都是有风险的,不建议。能量饮料对儿童、18岁以下的人、老年人、糖尿病患者、高血压患者、孕妇和哺乳期妇女、患有代谢疾病的人、肾衰竭以及对咖啡因敏感的人都有风险。近年来,在国内外,功能饮料的使用都在迅速增长。通常,年轻人、运动员和生活活跃的人都饮用能量饮料。能量饮料也被认为是一种乐趣,或者可以提高精神、身体和认知能力。本研究的目的是利用市场上常用的多种功能饮料的GC-MS(气相色谱-质谱分析)装置进行定性分析,分析其成分的含量和成分的有效性。此外,它是通过检查所确定的物质及其数量对人体健康的影响来评估市场上供应的能量饮料。
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 The aim of our study is to analyze the components of the content and the effectiveness of the components by making a qualitative analysis with the GC-MS (Gas Chromatography-Mass Analysis) device of many energy drinks used in the market. In addition, it is the evaluation of the energy drinks available in the market by examining the effects of the determined substances and their amounts on human health.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135431843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Capsaicin Shows Species and Strain-specific Activity: Investigation of the Antibacterial Effects on the Oral Pathogen Streptococcus mutans and the Oral Probiotics Streptococcus salivarius M18 and K12 辣椒素具有物种和菌株特异性活性:研究辣椒素对口腔病原体变异链球菌和口腔益生菌唾液链球菌 M18 和 K12 的抗菌作用
Pub Date : 2023-10-25 DOI: 10.15671/hjbc.1337284
Kader Doğan, Sinem Tunçer
Extensive research has investigated capsaicin (CAP), the primary bioactive compound in chili peppers, to explore its diverse pharmacological and physiological properties. Recently, the focus has shifted to understanding the potential effects of CAP on gut microbiota due to the strong link between gut bacterial profile and diet. However, there has been no research on the effects of CAP on oral microbiota. Therefore, our study aimed to explore the antibacterial effects of CAP on two oral probiotics, Streptococcus salivarius M18, and S. salivarius K12, along with the oral pathogen S. mutans. Previously, the anti-cancer activity of CAP had been demonstrated, and in accordance with these findings, here, we show its growth inhibitory activity on colorectal cancer cell lines. However, this study is the first to examine the impact of CAP on specific oral microorganisms while considering the oral consumption of CAP and the interconnectedness of the oral and gut microbiomes. The findings revealed that CAP exhibited antibacterial properties against the M18 strain at concentrations exceeding 100 µg/mL. Surprisingly, it did not show any growth-inhibitory effects on S. salivarius K12, even at a concentration of 2 mg/mL. Similarly, CAP did not inhibit the growth of S. mutans, a significant factor in dental caries. These results suggest that CAP's effects are species and strain-specific, indicating potential changes in the oral microbiota upon CAP consumption.
对辣椒中的主要生物活性化合物辣椒素(CAP)进行了广泛的研究,以探索其多种药理和生理特性。最近,研究重点转向了解 CAP 对肠道微生物群的潜在影响,因为肠道细菌谱与饮食之间存在密切联系。然而,目前还没有关于 CAP 对口腔微生物群影响的研究。因此,我们的研究旨在探索 CAP 对两种口腔益生菌(唾液链球菌 M18 和唾液链球菌 K12)以及口腔病原体 S. mutans 的抗菌作用。此前,CAP 的抗癌活性已得到证实,根据这些研究结果,我们在这里展示了它对大肠癌细胞株的生长抑制活性。然而,本研究是首次研究 CAP 对特定口腔微生物的影响,同时考虑到了口服 CAP 以及口腔和肠道微生物群之间的相互联系。研究结果表明,当浓度超过 100 µg/mL 时,CAP 对 M18 菌株具有抗菌特性。令人惊讶的是,即使在 2 毫克/毫升的浓度下,CAP 对唾液球菌 K12 也没有表现出任何生长抑制作用。同样,CAP 也没有抑制导致龋齿的一个重要因素--突变杆状病毒的生长。这些结果表明,CAP 的作用具有物种和菌株特异性,表明服用 CAP 后口腔微生物群可能会发生变化。
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引用次数: 0
Parental Care and Nest Site Properties in the European Roller, Coracias Garrulus in a Novel Breeding Site 一个新繁殖地的欧洲卷耳鹬的亲代抚育和巢地特性
Pub Date : 2023-10-25 DOI: 10.15671/hjbc.1342237
Necmiye ŞAHİN ARSLAN
I investigated parental behavior and nest site occupancy of the European Roller, Coracias garrulus in a novel breeding site, retaining walls with numerous embedded 10 cm diameter pipes in 2020–2022 in northern Turkey, Corum. I also searched the literature to review parental behavior and length of parental care in egg and nestling stages in other members of the order Coraciiformes. European Rollers occupied 6 holes for 12 nesting attempts across three years. Nest height from the ground was 4.7 ± 0.3 m (n = 12). I video-recorded the nest holes in the early morning during the incubation and nestling periods for 22.9 and 25 hours in total, respectively. Males and females shared incubation and nestling provisioning tasks. Nest attentiveness was 97.9% ± 0.79. Average feeding rate of nestlings was 10.86 ± 2.15 trips h-1. Feeding rates increased from early (5.94 ± 1.09 h-1) to late (15.8 ± 2.04 h-1) nestling ages, significantly. Ultimately, the comparisons among species in the order showed that the vast majority of species were relatively similar in development times but that little data is available on parental behavior. The latter emphasizes the great need for more study of parental behavior in this order.
2020-2022年,我在土耳其北部科鲁姆的一个新的繁殖地,有许多嵌入直径10厘米的管道的挡土墙上,调查了欧洲圆嘴鸦的亲代行为和巢址占用情况。我还检索了文献,回顾了其他喙形目成员在蛋和雏鸟阶段的亲代行为和亲代照顾的时间。欧洲辊子占用6个孔,在三年内进行了12次嵌套尝试。巢距地面高度为4.7±0.3 m (n = 12)。我分别在孵化期和雏鸟孵化期的清晨对巢洞进行了22.9小时和25小时的录像。雄性和雌性共同承担孵化和哺育幼雏的任务。巢内注意力为97.9%±0.79。平均采食率为10.86±2.15次h-1。采食率从雏鸟早期(5.94±1.09 h-1)到后期(15.8±2.04 h-1)显著升高。最终,该目物种之间的比较表明,绝大多数物种在发育时期相对相似,但关于亲代行为的数据很少。后者强调了在这个顺序中对父母行为进行更多研究的巨大需求。
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引用次数: 0
Glaucium Cappadocicum: Screening on Antioxidant, Antimicrobial, Anticholinesterase Inhibition in vitro 体外抗氧化、抗菌、抗胆碱酯酶抑制作用的筛选
Pub Date : 2023-10-19 DOI: 10.15671/hjbc.1332418
Sevgi ALTIN, Ekrem KÖKSAL
Glaucium cappadocicum is an endemic plant in Turkey, but there have been no research on it. In this work, the total phenolic and flavonoid content of extracts made from this plant were measured. Additionally, their antioxidant, antibacterial, and anticholinesterase activities were assessed. The extracts' antioxidant properties were assessed using the DPPH and FRAP methods, their antibacterial effects were assessed using the disk diffusion method on Gram-positive and Gram-negative bacteria, and their ability to inhibit cholinesterase activity was assessed using the Ellman method. In all biological activity assays, the alkaloid extract had the maximum activity. Alkaloid extract shown higher inhibition than the common inhibitor Tacrine in investigations on enzyme inhibition, in particular. All of these investigations demonstrate the potent anticholinesterase and antibacterial properties of Glaucium cappadocicum as well as its value as a good source of antioxidant system defense.
卡帕多卡姆(Glaucium cappadocicum)是土耳其特有植物,但目前尚无相关研究。测定了该植物提取物中总酚和总黄酮的含量。此外,还评估了它们的抗氧化、抗菌和抗胆碱酯酶活性。采用DPPH法和FRAP法测定提取物的抗氧化性能,采用圆盘扩散法测定提取物对革兰氏阳性菌和革兰氏阴性菌的抑菌作用,采用Ellman法测定提取物抑制胆碱酯酶活性的能力。在所有的生物活性测定中,生物碱提取物的活性最高。生物碱提取物对酶的抑制作用明显高于普通抑制剂他克林。这些研究都证明了卡帕多西蓝具有有效的抗胆碱酯酶和抗菌特性,以及作为抗氧化系统防御的良好来源的价值。
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引用次数: 0
Evaluation of Antifungal and Antiproliferative Effects of Two Different Organic Compounds with Cyclobutane Ring 两种含环丁烷的有机化合物的抗真菌和抗增殖作用的评价
Pub Date : 2023-07-05 DOI: 10.15671/hjbc.1311376
Mustafa Çiçek, T. Karaca, Ibrahim Yilmaz
In the modern world, where there is a growing demand for new substances with anticancer and antifungal activities, Schiff bases and phthalimide derivatives, which exhibit a wide diversity of biological activity, have become the focus of new therapeutic research studies. Accordingly, this study examined the anti-proliferative effects of two distinct compounds synthesized by cyclobutane substitution on breast cancer and liver cancer cell lines, which are two major cancer types, were investigated with MTT method and their antifugal activities on C. albicans were evaluated with disk diffusion method. Minimum inhibitory concentrations of the compounds against C. albicans were also determined in the scope of the study. The results revealed that the synthesized Schiff base was more effective on the breast cancer cell line MCF7, whereas the phthalimide-derivative was more effective against the liver cancer cell line Mahlavu. Besides, according to the data related to the antifungal properties of the compounds, it can be inferred that both compounds are suitable for further investigation as potential building blocks for the creation of novel and efficient antifungal medications.
现代世界对具有抗癌和抗真菌活性的新物质的需求日益增长,希夫碱和邻苯二甲酸亚胺衍生物具有广泛的生物活性,已成为新的治疗研究的焦点。因此,本研究采用MTT法研究了环丁烷取代法合成的两种不同的化合物对乳腺癌和肝癌两种主要癌症类型的抗增殖作用,并采用圆盘扩散法评价了它们对白色念珠菌的抗增殖活性。该化合物对白色念珠菌的最低抑制浓度也在研究范围内确定。结果表明,合成的希夫碱对乳腺癌细胞株MCF7更有效,而邻苯二胺衍生物对肝癌细胞株Mahlavu更有效。此外,根据化合物抗真菌特性的相关数据,可以推断这两种化合物都适合进一步研究,作为创建新型有效抗真菌药物的潜在构建块。
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引用次数: 0
Investigation of the Siderophore Production and Associated Heavy Metal Accumulation Potential of Brevibacillus laterosporus 301/İK3-2 侧芽孢短杆菌301/İK3-2铁载体产量及相关重金属积累潜力的研究
Pub Date : 2023-06-08 DOI: 10.15671/hjbc.1256836
F. I. Özdemir, Bahadır Aydin, A. Tülek
Siderophores are secondary metabolites released into the environment by various microorganisms, fungi, and plants to chelate iron from the surrounding environment. It is known that siderophores bind to other metals besides iron. Today, heavy metals, which are released as an undesirable result of industrial development, accumulate at high rates and pose a significant threat to biological living things. In this sense, remediation of heavy metal-contaminated sites is an urgent requirement. Siderophores are promising agents for the removal of heavy metals from natural habitats with the role of bioremediation. In this study, the effect of heavy metals on the growth and siderophore production of Brevibacillus laterosporus was investigated. Maximum siderophore production was determined as 50 % at 48 h in the metal-free growth media. In addition, maximum siderophore production was determined for various heavy metals including 5 μM Hg2+, 0.5 mM Ni2+, 0.1 mM Co2+, and 2.5 μM Fe2+. Intracellular uptake of the mercury was also measured using optical emission spectroscopy and compared with siderophore production values of the B. laterosporus. The maximum biosorption of mercury was measured to be 40% in 5 μM Hg2+-containing media at 48 h of incubation. The results show that siderophore production is affected by uptake of various metals, and are usable for removing of heavy metals from environmental habitats.
铁载体是由各种微生物、真菌和植物释放到环境中的次级代谢物,用于螯合周围环境中的铁。众所周知,铁载体还能与铁以外的其他金属结合。今天,重金属作为工业发展的不良后果被释放出来,它以高速度积累,对生物生物构成重大威胁。从这个意义上说,修复重金属污染场地是一个迫切的要求。铁载体是一种具有生物修复作用的重金属脱除剂。本研究研究了重金属对晚孢短芽孢杆菌生长和产铁载体的影响。在无金属生长培养基中,48 h最大铁载体产量为50%。此外,在5 μM Hg2+、0.5 mM Ni2+、0.1 mM Co2+和2.5 μM Fe2+等重金属环境下,铁载体的最大产量也被确定。还利用光学发射光谱测量了细胞内汞的摄取,并与侧芽孢杆菌的铁载体产生值进行了比较。在含5 μM Hg2+的培养基中,培养48 h,汞的最大生物吸附性为40%。结果表明,铁载体的产生受各种金属的吸收影响,可用于去除环境生境中的重金属。
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引用次数: 0
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Hacettepe Journal of Biology and Chemistry
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