Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone (PCL), hyaluronic acid (HA) coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent (IF) staining was performed with glial fibrillary acidic protein (GFAP) antibody to determine the level of gliosis. It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.
{"title":"COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS","authors":"Ece BATTALOĞLU, Zehra Gül MORÇİMEN, Aylin ŞENDEMİR","doi":"10.15671/hjbc.1245678","DOIUrl":"https://doi.org/10.15671/hjbc.1245678","url":null,"abstract":"Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone (PCL), hyaluronic acid (HA) coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent (IF) staining was performed with glial fibrillary acidic protein (GFAP) antibody to determine the level of gliosis. It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"20 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135432020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The mechanism of development of chronic rhinosinusitis(CRS) is not fully known. However, bacteria are thought to play an important role in this clinic. It has been suggested that toxins with superantigen(SAgs) properties produced by one of these bacteria, Staphylococcus aureus(S.aureus), play a role in the development of inflammation associated with sinusitis. In this study, S.aureus was isolated by taking endoscopic sinus biopsy samples and nasal swab samples from patients with CRS and the control group. It was aimed to examine the frequency of S.aureus presence in the samples taken, the presence of toxin genes showing superantigen quality in these isolated bacteria, and to evaluate the roles of these parameters in the development of CRS. More S.aureus was isolated in the samples taken from patients with CRS than in the control group. The isolated S.aureus samples were analysed by real-time PCR method. The presence of enterotoxin A, B, C and D genes in the S.aureus samples isolated from the patient group were found at the rates of 54%, 32%, 16% and 16%, respectively, while these rates were 46%, 24%, 14% and 14% in the control group. The Toxic Shock Syndrome Toxin-1(TSST-1) gene was detected in 20% of the samples isolated from the patient and 46% in the control group bacteria. The fact that S.aureus was isolated in 20% of the patients shows that this bacterium is not necessary for CRS. The frequency of superantigen toxin genes in S.aureus isolates shows that these toxins are not necessary for the development of the disease.
{"title":"Assessment of Staphylococcal toxins acting as superantigens in different nasal specimens in the etiology of chronic rhinosinusitis","authors":"Gülden VURAL, Alper CEYLAN, M. Nedim SULTAN","doi":"10.15671/hjbc.1302331","DOIUrl":"https://doi.org/10.15671/hjbc.1302331","url":null,"abstract":"The mechanism of development of chronic rhinosinusitis(CRS) is not fully known. However, bacteria are thought to play an important role in this clinic. It has been suggested that toxins with superantigen(SAgs) properties produced by one of these bacteria, Staphylococcus aureus(S.aureus), play a role in the development of inflammation associated with sinusitis. In this study, S.aureus was isolated by taking endoscopic sinus biopsy samples and nasal swab samples from patients with CRS and the control group. It was aimed to examine the frequency of S.aureus presence in the samples taken, the presence of toxin genes showing superantigen quality in these isolated bacteria, and to evaluate the roles of these parameters in the development of CRS. More S.aureus was isolated in the samples taken from patients with CRS than in the control group. The isolated S.aureus samples were analysed by real-time PCR method. The presence of enterotoxin A, B, C and D genes in the S.aureus samples isolated from the patient group were found at the rates of 54%, 32%, 16% and 16%, respectively, while these rates were 46%, 24%, 14% and 14% in the control group. The Toxic Shock Syndrome Toxin-1(TSST-1) gene was detected in 20% of the samples isolated from the patient and 46% in the control group bacteria. The fact that S.aureus was isolated in 20% of the patients shows that this bacterium is not necessary for CRS. The frequency of superantigen toxin genes in S.aureus isolates shows that these toxins are not necessary for the development of the disease.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"314 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135474790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cellulosic wastes constitute the majority of agricultural fields. The purpose of this study was to utilize these cellulosic wastes such as wheat straw and wheat straw cellulose on soil carbon mineralization in a sandy loam soils. Two different doses (100 and 1000 mg) of wheat straw cellulose were used to determine the carbon mineralization using CO2 respiration method. The cumulative carbon mineralization was found to be highest at the minumum doses of wheat straw cellulose with nitrogen (W-CL-N, 19.65 mg) and the lowest at the maximum doses of wheat straw (W-Straw, 14.32 mg). The results showed that the application of wheat straw cellulose at minumum doses resulted in higher carbon mineralization rate. The maximum carbon mineralization rate was observed in soil with minimum wheat straw cellulose and nitrogen source were added (1.41%). Whereas, the minimum carbon mineralization rate was determined in the soil mixed maximum wheat straw (1.03 %). The soil mixed maximum wheat straw was determined the lowest carbon mineralization rate due to its complex structure. The use of nitrogen source and organic matter with cellulose have positive effect on soil carbon mineralization. It might be said that these results describe an effective way for disposal of organic wastes.
{"title":"THE SHORT-TERM EFFECTS OF WHEAT STRAW CELLULOSE ON SOIL CARBON MINERALIZATION","authors":"Bahar MERYEMOĞLU, Nacide KIZILDAĞ ÖZDAL","doi":"10.15671/hjbc.1270451","DOIUrl":"https://doi.org/10.15671/hjbc.1270451","url":null,"abstract":"Cellulosic wastes constitute the majority of agricultural fields. The purpose of this study was to utilize these cellulosic wastes such as wheat straw and wheat straw cellulose on soil carbon mineralization in a sandy loam soils. Two different doses (100 and 1000 mg) of wheat straw cellulose were used to determine the carbon mineralization using CO2 respiration method. The cumulative carbon mineralization was found to be highest at the minumum doses of wheat straw cellulose with nitrogen (W-CL-N, 19.65 mg) and the lowest at the maximum doses of wheat straw (W-Straw, 14.32 mg). The results showed that the application of wheat straw cellulose at minumum doses resulted in higher carbon mineralization rate. The maximum carbon mineralization rate was observed in soil with minimum wheat straw cellulose and nitrogen source were added (1.41%). Whereas, the minimum carbon mineralization rate was determined in the soil mixed maximum wheat straw (1.03 %). The soil mixed maximum wheat straw was determined the lowest carbon mineralization rate due to its complex structure. The use of nitrogen source and organic matter with cellulose have positive effect on soil carbon mineralization. It might be said that these results describe an effective way for disposal of organic wastes.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"313 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135474792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The detection of foodborne pathogenic bacteria remains a significant challenge, and the need for fast and sensitive detection methods is becoming increasingly important. Escherichia coli is a prevalent bacteria associated with foodborne illness, and this study aimed to evaluate the ability of a surface plasmon resonance (SPR) based biosensor to detect E. coli O157:H7 at low levels in pure culture and artificially contaminated bay leaves (Laurus nobilis) using different injection methods. To develop a biological sensing surface, the sensor surface was functionalized with 3-aminopropyltriethoxysilane (APTES), and polyclonal antibodies were immobilized on the surface for bacteria detection. Bacterial attachment to the antibodies resulted in a change in resonance angle. The biosensor was able to discriminate between cellular concentrations of 103 to 107 CFU/mL and showed potential in detecting different pathogens in various food samples. Before the SPR detection, the sample preparation step was optimized to ensure complex food matrices were suitable for SPR analysis. The results suggest that the SPR based biosensor is a promising tool for the rapid detection of foodborne pathogens in complex food matrices.
{"title":"E. coli O157:H7 Detection Using Surface Plasmon Resonance Based Biosensor","authors":"Esma ESER, Okan Öner EKİZ, H. İbrahim EKİZ","doi":"10.15671/hjbc.1271685","DOIUrl":"https://doi.org/10.15671/hjbc.1271685","url":null,"abstract":"The detection of foodborne pathogenic bacteria remains a significant challenge, and the need for fast and sensitive detection methods is becoming increasingly important. Escherichia coli is a prevalent bacteria associated with foodborne illness, and this study aimed to evaluate the ability of a surface plasmon resonance (SPR) based biosensor to detect E. coli O157:H7 at low levels in pure culture and artificially contaminated bay leaves (Laurus nobilis) using different injection methods. To develop a biological sensing surface, the sensor surface was functionalized with 3-aminopropyltriethoxysilane (APTES), and polyclonal antibodies were immobilized on the surface for bacteria detection. Bacterial attachment to the antibodies resulted in a change in resonance angle. The biosensor was able to discriminate between cellular concentrations of 103 to 107 CFU/mL and showed potential in detecting different pathogens in various food samples. Before the SPR detection, the sample preparation step was optimized to ensure complex food matrices were suitable for SPR analysis. The results suggest that the SPR based biosensor is a promising tool for the rapid detection of foodborne pathogens in complex food matrices.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"313 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135474791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the Turkish Food Codex Communique on Energy Drinks, energy drinks are defined as "flavored non-alcoholic beverage containing caffeine, containing taurine, glucuronolactone, inositol, carbohydrates, amino acids, vitamins, minerals, and other food and components". The amount of caffeine in the composition of the energy drink cannot be more than 150 mg/L, inositol 100 mg/L, glucuronolactone 20 mg/L, and taurine 800 mg/L. It has been reported that the consumption of beverages containing caffeine, taurine, and glucuronolactone in healthy young adults increases arterial blood pressure and platelet aggregation and decreases endothelial function. For healthy adults, consuming more than 400 mg of caffeine daily and consuming more than 500 ml of energy drinks in energy drinks, as well as consuming energy drinks with or together with alcohol, is risky and not recommended. Energy drinks are risky for children, people under the age of 18, the elderly, diabetics, those with high blood pressure, pregnant and lactating women, those with metabolic diseases, kidney failure, and people who are sensitive to caffeine. The use of energy drinks has been increasing rapidly in the world and in our country in recent years. Usually, energy drinks are used by young people, athletes, and people who live actively. Energy drinks are also preferred as a pleasure or to increase mental, physical, and cognitive performance.
The aim of our study is to analyze the components of the content and the effectiveness of the components by making a qualitative analysis with the GC-MS (Gas Chromatography-Mass Analysis) device of many energy drinks used in the market. In addition, it is the evaluation of the energy drinks available in the market by examining the effects of the determined substances and their amounts on human health.
{"title":"Content Analysis of Locally Marketed Energy Drinks: Turkish Market","authors":"Zafer PEKER, Dursun Ali KÖSE","doi":"10.15671/hjbc.1279376","DOIUrl":"https://doi.org/10.15671/hjbc.1279376","url":null,"abstract":"In the Turkish Food Codex Communique on Energy Drinks, energy drinks are defined as \"flavored non-alcoholic beverage containing caffeine, containing taurine, glucuronolactone, inositol, carbohydrates, amino acids, vitamins, minerals, and other food and components\". The amount of caffeine in the composition of the energy drink cannot be more than 150 mg/L, inositol 100 mg/L, glucuronolactone 20 mg/L, and taurine 800 mg/L. It has been reported that the consumption of beverages containing caffeine, taurine, and glucuronolactone in healthy young adults increases arterial blood pressure and platelet aggregation and decreases endothelial function. For healthy adults, consuming more than 400 mg of caffeine daily and consuming more than 500 ml of energy drinks in energy drinks, as well as consuming energy drinks with or together with alcohol, is risky and not recommended. Energy drinks are risky for children, people under the age of 18, the elderly, diabetics, those with high blood pressure, pregnant and lactating women, those with metabolic diseases, kidney failure, and people who are sensitive to caffeine. The use of energy drinks has been increasing rapidly in the world and in our country in recent years. Usually, energy drinks are used by young people, athletes, and people who live actively. Energy drinks are also preferred as a pleasure or to increase mental, physical, and cognitive performance.
 The aim of our study is to analyze the components of the content and the effectiveness of the components by making a qualitative analysis with the GC-MS (Gas Chromatography-Mass Analysis) device of many energy drinks used in the market. In addition, it is the evaluation of the energy drinks available in the market by examining the effects of the determined substances and their amounts on human health.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135431843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Extensive research has investigated capsaicin (CAP), the primary bioactive compound in chili peppers, to explore its diverse pharmacological and physiological properties. Recently, the focus has shifted to understanding the potential effects of CAP on gut microbiota due to the strong link between gut bacterial profile and diet. However, there has been no research on the effects of CAP on oral microbiota. Therefore, our study aimed to explore the antibacterial effects of CAP on two oral probiotics, Streptococcus salivarius M18, and S. salivarius K12, along with the oral pathogen S. mutans. Previously, the anti-cancer activity of CAP had been demonstrated, and in accordance with these findings, here, we show its growth inhibitory activity on colorectal cancer cell lines. However, this study is the first to examine the impact of CAP on specific oral microorganisms while considering the oral consumption of CAP and the interconnectedness of the oral and gut microbiomes. The findings revealed that CAP exhibited antibacterial properties against the M18 strain at concentrations exceeding 100 µg/mL. Surprisingly, it did not show any growth-inhibitory effects on S. salivarius K12, even at a concentration of 2 mg/mL. Similarly, CAP did not inhibit the growth of S. mutans, a significant factor in dental caries. These results suggest that CAP's effects are species and strain-specific, indicating potential changes in the oral microbiota upon CAP consumption.
对辣椒中的主要生物活性化合物辣椒素（CAP）进行了广泛的研究，以探索其多种药理和生理特性。最近，研究重点转向了解 CAP 对肠道微生物群的潜在影响，因为肠道细菌谱与饮食之间存在密切联系。然而，目前还没有关于 CAP 对口腔微生物群影响的研究。因此，我们的研究旨在探索 CAP 对两种口腔益生菌（唾液链球菌 M18 和唾液链球菌 K12）以及口腔病原体 S. mutans 的抗菌作用。此前，CAP 的抗癌活性已得到证实，根据这些研究结果，我们在这里展示了它对大肠癌细胞株的生长抑制活性。然而，本研究是首次研究 CAP 对特定口腔微生物的影响，同时考虑到了口服 CAP 以及口腔和肠道微生物群之间的相互联系。研究结果表明，当浓度超过 100 µg/mL 时，CAP 对 M18 菌株具有抗菌特性。令人惊讶的是，即使在 2 毫克/毫升的浓度下，CAP 对唾液球菌 K12 也没有表现出任何生长抑制作用。同样，CAP 也没有抑制导致龋齿的一个重要因素--突变杆状病毒的生长。这些结果表明，CAP 的作用具有物种和菌株特异性，表明服用 CAP 后口腔微生物群可能会发生变化。
{"title":"Capsaicin Shows Species and Strain-specific Activity: Investigation of the Antibacterial Effects on the Oral Pathogen Streptococcus mutans and the Oral Probiotics Streptococcus salivarius M18 and K12","authors":"Kader Doğan, Sinem Tunçer","doi":"10.15671/hjbc.1337284","DOIUrl":"https://doi.org/10.15671/hjbc.1337284","url":null,"abstract":"Extensive research has investigated capsaicin (CAP), the primary bioactive compound in chili peppers, to explore its diverse pharmacological and physiological properties. Recently, the focus has shifted to understanding the potential effects of CAP on gut microbiota due to the strong link between gut bacterial profile and diet. However, there has been no research on the effects of CAP on oral microbiota. Therefore, our study aimed to explore the antibacterial effects of CAP on two oral probiotics, Streptococcus salivarius M18, and S. salivarius K12, along with the oral pathogen S. mutans. Previously, the anti-cancer activity of CAP had been demonstrated, and in accordance with these findings, here, we show its growth inhibitory activity on colorectal cancer cell lines. However, this study is the first to examine the impact of CAP on specific oral microorganisms while considering the oral consumption of CAP and the interconnectedness of the oral and gut microbiomes. The findings revealed that CAP exhibited antibacterial properties against the M18 strain at concentrations exceeding 100 µg/mL. Surprisingly, it did not show any growth-inhibitory effects on S. salivarius K12, even at a concentration of 2 mg/mL. Similarly, CAP did not inhibit the growth of S. mutans, a significant factor in dental caries. These results suggest that CAP's effects are species and strain-specific, indicating potential changes in the oral microbiota upon CAP consumption.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139314327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I investigated parental behavior and nest site occupancy of the European Roller, Coracias garrulus in a novel breeding site, retaining walls with numerous embedded 10 cm diameter pipes in 2020–2022 in northern Turkey, Corum. I also searched the literature to review parental behavior and length of parental care in egg and nestling stages in other members of the order Coraciiformes. European Rollers occupied 6 holes for 12 nesting attempts across three years. Nest height from the ground was 4.7 ± 0.3 m (n = 12). I video-recorded the nest holes in the early morning during the incubation and nestling periods for 22.9 and 25 hours in total, respectively. Males and females shared incubation and nestling provisioning tasks. Nest attentiveness was 97.9% ± 0.79. Average feeding rate of nestlings was 10.86 ± 2.15 trips h-1. Feeding rates increased from early (5.94 ± 1.09 h-1) to late (15.8 ± 2.04 h-1) nestling ages, significantly. Ultimately, the comparisons among species in the order showed that the vast majority of species were relatively similar in development times but that little data is available on parental behavior. The latter emphasizes the great need for more study of parental behavior in this order.
2020-2022年，我在土耳其北部科鲁姆的一个新的繁殖地，有许多嵌入直径10厘米的管道的挡土墙上，调查了欧洲圆嘴鸦的亲代行为和巢址占用情况。我还检索了文献，回顾了其他喙形目成员在蛋和雏鸟阶段的亲代行为和亲代照顾的时间。欧洲辊子占用6个孔，在三年内进行了12次嵌套尝试。巢距地面高度为4.7±0.3 m (n = 12)。我分别在孵化期和雏鸟孵化期的清晨对巢洞进行了22.9小时和25小时的录像。雄性和雌性共同承担孵化和哺育幼雏的任务。巢内注意力为97.9%±0.79。平均采食率为10.86±2.15次h-1。采食率从雏鸟早期(5.94±1.09 h-1)到后期(15.8±2.04 h-1)显著升高。最终，该目物种之间的比较表明，绝大多数物种在发育时期相对相似，但关于亲代行为的数据很少。后者强调了在这个顺序中对父母行为进行更多研究的巨大需求。
{"title":"Parental Care and Nest Site Properties in the European Roller, Coracias Garrulus in a Novel Breeding Site","authors":"Necmiye ŞAHİN ARSLAN","doi":"10.15671/hjbc.1342237","DOIUrl":"https://doi.org/10.15671/hjbc.1342237","url":null,"abstract":"I investigated parental behavior and nest site occupancy of the European Roller, Coracias garrulus in a novel breeding site, retaining walls with numerous embedded 10 cm diameter pipes in 2020–2022 in northern Turkey, Corum. I also searched the literature to review parental behavior and length of parental care in egg and nestling stages in other members of the order Coraciiformes. European Rollers occupied 6 holes for 12 nesting attempts across three years. Nest height from the ground was 4.7 ± 0.3 m (n = 12). I video-recorded the nest holes in the early morning during the incubation and nestling periods for 22.9 and 25 hours in total, respectively. Males and females shared incubation and nestling provisioning tasks. Nest attentiveness was 97.9% ± 0.79. Average feeding rate of nestlings was 10.86 ± 2.15 trips h-1. Feeding rates increased from early (5.94 ± 1.09 h-1) to late (15.8 ± 2.04 h-1) nestling ages, significantly. Ultimately, the comparisons among species in the order showed that the vast majority of species were relatively similar in development times but that little data is available on parental behavior. The latter emphasizes the great need for more study of parental behavior in this order.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glaucium cappadocicum is an endemic plant in Turkey, but there have been no research on it. In this work, the total phenolic and flavonoid content of extracts made from this plant were measured. Additionally, their antioxidant, antibacterial, and anticholinesterase activities were assessed. The extracts' antioxidant properties were assessed using the DPPH and FRAP methods, their antibacterial effects were assessed using the disk diffusion method on Gram-positive and Gram-negative bacteria, and their ability to inhibit cholinesterase activity was assessed using the Ellman method. In all biological activity assays, the alkaloid extract had the maximum activity. Alkaloid extract shown higher inhibition than the common inhibitor Tacrine in investigations on enzyme inhibition, in particular. All of these investigations demonstrate the potent anticholinesterase and antibacterial properties of Glaucium cappadocicum as well as its value as a good source of antioxidant system defense.
{"title":"Glaucium Cappadocicum: Screening on Antioxidant, Antimicrobial, Anticholinesterase Inhibition in vitro","authors":"Sevgi ALTIN, Ekrem KÖKSAL","doi":"10.15671/hjbc.1332418","DOIUrl":"https://doi.org/10.15671/hjbc.1332418","url":null,"abstract":"Glaucium cappadocicum is an endemic plant in Turkey, but there have been no research on it. In this work, the total phenolic and flavonoid content of extracts made from this plant were measured. Additionally, their antioxidant, antibacterial, and anticholinesterase activities were assessed. The extracts' antioxidant properties were assessed using the DPPH and FRAP methods, their antibacterial effects were assessed using the disk diffusion method on Gram-positive and Gram-negative bacteria, and their ability to inhibit cholinesterase activity was assessed using the Ellman method. In all biological activity assays, the alkaloid extract had the maximum activity. Alkaloid extract shown higher inhibition than the common inhibitor Tacrine in investigations on enzyme inhibition, in particular. All of these investigations demonstrate the potent anticholinesterase and antibacterial properties of Glaucium cappadocicum as well as its value as a good source of antioxidant system defense.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135823097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the modern world, where there is a growing demand for new substances with anticancer and antifungal activities, Schiff bases and phthalimide derivatives, which exhibit a wide diversity of biological activity, have become the focus of new therapeutic research studies. Accordingly, this study examined the anti-proliferative effects of two distinct compounds synthesized by cyclobutane substitution on breast cancer and liver cancer cell lines, which are two major cancer types, were investigated with MTT method and their antifugal activities on C. albicans were evaluated with disk diffusion method. Minimum inhibitory concentrations of the compounds against C. albicans were also determined in the scope of the study. The results revealed that the synthesized Schiff base was more effective on the breast cancer cell line MCF7, whereas the phthalimide-derivative was more effective against the liver cancer cell line Mahlavu. Besides, according to the data related to the antifungal properties of the compounds, it can be inferred that both compounds are suitable for further investigation as potential building blocks for the creation of novel and efficient antifungal medications.
{"title":"Evaluation of Antifungal and Antiproliferative Effects of Two Different Organic Compounds with Cyclobutane Ring","authors":"Mustafa Çiçek, T. Karaca, Ibrahim Yilmaz","doi":"10.15671/hjbc.1311376","DOIUrl":"https://doi.org/10.15671/hjbc.1311376","url":null,"abstract":"In the modern world, where there is a growing demand for new substances with anticancer and antifungal activities, Schiff bases and phthalimide derivatives, which exhibit a wide diversity of biological activity, have become the focus of new therapeutic research studies. Accordingly, this study examined the anti-proliferative effects of two distinct compounds synthesized by cyclobutane substitution on breast cancer and liver cancer cell lines, which are two major cancer types, were investigated with MTT method and their antifugal activities on C. albicans were evaluated with disk diffusion method. Minimum inhibitory concentrations of the compounds against C. albicans were also determined in the scope of the study. The results revealed that the synthesized Schiff base was more effective on the breast cancer cell line MCF7, whereas the phthalimide-derivative was more effective against the liver cancer cell line Mahlavu. Besides, according to the data related to the antifungal properties of the compounds, it can be inferred that both compounds are suitable for further investigation as potential building blocks for the creation of novel and efficient antifungal medications.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88542337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Siderophores are secondary metabolites released into the environment by various microorganisms, fungi, and plants to chelate iron from the surrounding environment. It is known that siderophores bind to other metals besides iron. Today, heavy metals, which are released as an undesirable result of industrial development, accumulate at high rates and pose a significant threat to biological living things. In this sense, remediation of heavy metal-contaminated sites is an urgent requirement. Siderophores are promising agents for the removal of heavy metals from natural habitats with the role of bioremediation. In this study, the effect of heavy metals on the growth and siderophore production of Brevibacillus laterosporus was investigated. Maximum siderophore production was determined as 50 % at 48 h in the metal-free growth media. In addition, maximum siderophore production was determined for various heavy metals including 5 μM Hg2+, 0.5 mM Ni2+, 0.1 mM Co2+, and 2.5 μM Fe2+. Intracellular uptake of the mercury was also measured using optical emission spectroscopy and compared with siderophore production values of the B. laterosporus. The maximum biosorption of mercury was measured to be 40% in 5 μM Hg2+-containing media at 48 h of incubation. The results show that siderophore production is affected by uptake of various metals, and are usable for removing of heavy metals from environmental habitats.
铁载体是由各种微生物、真菌和植物释放到环境中的次级代谢物，用于螯合周围环境中的铁。众所周知，铁载体还能与铁以外的其他金属结合。今天，重金属作为工业发展的不良后果被释放出来，它以高速度积累，对生物生物构成重大威胁。从这个意义上说，修复重金属污染场地是一个迫切的要求。铁载体是一种具有生物修复作用的重金属脱除剂。本研究研究了重金属对晚孢短芽孢杆菌生长和产铁载体的影响。在无金属生长培养基中，48 h最大铁载体产量为50%。此外，在5 μM Hg2+、0.5 mM Ni2+、0.1 mM Co2+和2.5 μM Fe2+等重金属环境下，铁载体的最大产量也被确定。还利用光学发射光谱测量了细胞内汞的摄取，并与侧芽孢杆菌的铁载体产生值进行了比较。在含5 μM Hg2+的培养基中，培养48 h，汞的最大生物吸附性为40%。结果表明，铁载体的产生受各种金属的吸收影响，可用于去除环境生境中的重金属。
{"title":"Investigation of the Siderophore Production and Associated Heavy Metal Accumulation Potential of Brevibacillus laterosporus 301/İK3-2","authors":"F. I. Özdemir, Bahadır Aydin, A. Tülek","doi":"10.15671/hjbc.1256836","DOIUrl":"https://doi.org/10.15671/hjbc.1256836","url":null,"abstract":"Siderophores are secondary metabolites released into the environment by various microorganisms, fungi, and plants to chelate iron from the surrounding environment. It is known that siderophores bind to other metals besides iron. Today, heavy metals, which are released as an undesirable result of industrial development, accumulate at high rates and pose a significant threat to biological living things. In this sense, remediation of heavy metal-contaminated sites is an urgent requirement. Siderophores are promising agents for the removal of heavy metals from natural habitats with the role of bioremediation. \u0000In this study, the effect of heavy metals on the growth and siderophore production of Brevibacillus laterosporus was investigated. Maximum siderophore production was determined as 50 % at 48 h in the metal-free growth media. In addition, maximum siderophore production was determined for various heavy metals including 5 μM Hg2+, 0.5 mM Ni2+, 0.1 mM Co2+, and 2.5 μM Fe2+. Intracellular uptake of the mercury was also measured using optical emission spectroscopy and compared with siderophore production values of the B. laterosporus. The maximum biosorption of mercury was measured to be 40% in 5 μM Hg2+-containing media at 48 h of incubation. The results show that siderophore production is affected by uptake of various metals, and are usable for removing of heavy metals from environmental habitats.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78825017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：