Timing of methamphetamine exposure during adolescence differentially influences parvalbumin and perineuronal net immunoreactivity in the medial prefrontal cortex of female, but not male, rats.

IF 3 4区 材料科学 Q3 CHEMISTRY, PHYSICAL Solid State Ionics Pub Date : 2024-02-28 DOI:10.1101/2023.08.25.554911
Amara S Brinks, Lauren K Carrica, Dominic J Tagler, Joshua M Gulley, Janice M Juraska
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Abstract

Adolescence involves significant reorganization within the medial prefrontal cortex (mPFC), including modifications to inhibitory neurotransmission mediated through parvalbumin (PV) interneurons and their surrounding perineuronal nets (PNNs). These developmental changes, which result in increased PV neuron activity in adulthood, may be disrupted by drug use resulting in lasting changes in mPFC function and behavior. Methamphetamine (METH), which is a readily available drug used by some adolescents, increases PV neuron activity and could influence the activity-dependent maturational process of these neurons. In the present study, we used male and female Sprague Dawley rats to test the hypothesis that METH exposure influences PV and PNN expression in a sex- and age-specific manner. Rats were injected daily with saline or 3.0 mg/kg METH from early adolescence (EA; 30-38 days old), late adolescence (LA; 40-48 days old), or young adulthood (60-68 days old). One day following exposure, effects of METH on PV cell and PNN expression were assessed using immunofluorescent labeling within the mPFC. METH exposure did not alter male PV neurons or PNNs. Females exposed in early adolescence or adulthood had more PV expressing neurons while those exposed in later adolescence had fewer, suggesting distinct windows of vulnerability to changes induced by METH exposure. In addition, females exposed to METH had more PNNs and more intense PV neuron staining, further suggesting that METH exposure in adolescence uniquely influences development of inhibitory circuits in the female mPFC. This study indicates that the timing of METH exposure, even within adolescence, influences its neural effects in females.

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青春期接触甲基苯丙胺的时间会对雌性大鼠内侧前额叶皮层的副视蛋白和神经元周围网免疫反应产生不同影响,而对雄性大鼠则无影响。
青春期涉及内侧前额叶皮层(mPFC)内的重大重组,包括通过副缬氨酸(PV)中间神经元及其周围神经元网络(PNN)介导的抑制性神经递质的改变。这些发育变化会导致副视神经元的活动在成年后增加,但吸毒可能会破坏这些变化,从而导致 mPFC 功能和行为的持久变化。甲基苯丙胺(METH)是一些青少年经常使用的一种毒品,它能增加PV神经元的活动,并可能影响这些神经元依赖活动的成熟过程。在本研究中,我们使用雄性和雌性 Sprague Dawley 大鼠来验证 METH 暴露以性别和年龄特异性的方式影响 PV 和 PNN 表达的假设。从青春期早期(EA;30-38 天)、青春期晚期(LA;40-48 天)或青年期(60-68 天)开始,每天给大鼠注射生理盐水或 3.0 mg/kg METH。暴露一天后,在 mPFC 中使用免疫荧光标记评估 METH 对 PV 细胞和 PNN 表达的影响。暴露于 METH 不会改变雄性 PV 神经元或 PNN。暴露于早期青春期或成年期的女性有更多的 PV 表达神经元,而暴露于晚期青春期的女性则较少,这表明暴露于 METH 引起的变化有不同的易感窗口期。此外,暴露于 METH 的女性有更多的 PNN 和更强烈的 PV 神经元染色,这进一步表明,青春期暴露于 METH 会独特地影响女性 mPFC 抑制回路的发育。这项研究表明,暴露于 METH 的时间,即使是在青春期,也会影响其对女性神经的影响。
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来源期刊
Solid State Ionics
Solid State Ionics 物理-物理:凝聚态物理
CiteScore
6.10
自引率
3.10%
发文量
152
审稿时长
58 days
期刊介绍: This interdisciplinary journal is devoted to the physics, chemistry and materials science of diffusion, mass transport, and reactivity of solids. The major part of each issue is devoted to articles on: (i) physics and chemistry of defects in solids; (ii) reactions in and on solids, e.g. intercalation, corrosion, oxidation, sintering; (iii) ion transport measurements, mechanisms and theory; (iv) solid state electrochemistry; (v) ionically-electronically mixed conducting solids. Related technological applications are also included, provided their characteristics are interpreted in terms of the basic solid state properties. Review papers and relevant symposium proceedings are welcome.
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