Pawan Deep Singh, Lalit Dutta, Onkar Singh, Sanjeev Mishra, A. Khuroo, T. Monif, A. Ahmed, Nirmala Yadav
{"title":"Selective, sensitive, and rapid liquid chromatography-tandem mass spectrometry method for determination of Guaifenesin in human plasma","authors":"Pawan Deep Singh, Lalit Dutta, Onkar Singh, Sanjeev Mishra, A. Khuroo, T. Monif, A. Ahmed, Nirmala Yadav","doi":"10.3109/10601333.2010.513387","DOIUrl":null,"url":null,"abstract":"A simple, sensitive, selective, and rapid liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for quantitation of guaifenesin in human plasma using guaifenesin-d3 as internal standard (IS). Following solid phase extraction, the analyte was chromatographed using an isocratic mobile phase on a reversed-phase C18 column and analyzed by MS in multiple reaction-monitoring (MRM) mode (positive ion mode). The limit of quantitation for this method was 8 ng/mL and the linear dynamic range was 8–2200 ng/mL.","PeriodicalId":10446,"journal":{"name":"Clinical Research and Regulatory Affairs","volume":"52 1","pages":"121 - 127"},"PeriodicalIF":0.0000,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Research and Regulatory Affairs","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10601333.2010.513387","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A simple, sensitive, selective, and rapid liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for quantitation of guaifenesin in human plasma using guaifenesin-d3 as internal standard (IS). Following solid phase extraction, the analyte was chromatographed using an isocratic mobile phase on a reversed-phase C18 column and analyzed by MS in multiple reaction-monitoring (MRM) mode (positive ion mode). The limit of quantitation for this method was 8 ng/mL and the linear dynamic range was 8–2200 ng/mL.
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