AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JERUK PURUT (Citrus hystrix) TERHADAP BAKTERI Salmonella typhi

Yoga Alim Prisnanda, D. Wulandari
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Abstract

The lime leaves (Citrus hystrix) containing alkaloids, flavonoids, saponins and tannins which have antibacterial activity. The aims of the research was to determine the antibacterial effect of lime leaf extract to against Salmonella typhi bacteria. This research was initiated with extracting lime leaves, testing the chemical content of lime leaf extract, identifying bacteria, and testing the antibacterial activity of lime leaf extract using the disc diffusion method. The concentration of extract to used antibacterial activity test with diffusion methode was 2.5%; 5%; 10%. Positive control was chloramphenicol and negative control was DMSO 8%. based on kaffir lime leaf extract test, positively contained flavonoids, alkaloid tannins and saponins. Kaffir lime leaf extract has antibacterial activity for Salmonella thypi bacteria. The antibacterial activity test showed an average inhibition zone of 8 mm at a concentration of 2.5%, 9.6 mm at a concentration of 5% and 11.6 mm at a concentration of 10%.
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红斑柑橘叶乙醇提取物对沙门氏菌的抗菌活性
酸橙叶(Citrus hystrix)含有生物碱、类黄酮、皂苷和单宁,具有抗菌活性。研究青柠叶提取物对伤寒沙门氏菌的抑菌作用。本研究从提取石灰叶、测定石灰叶提取物的化学成分、鉴定细菌、采用圆盘扩散法测定石灰叶提取物的抑菌活性开始。采用扩散法进行抗菌活性试验,提取液浓度为2.5%;5%;10%. 阳性对照为氯霉素,阴性对照为DMSO 8%。酸橙叶提取物试验表明,其主要成分为黄酮类化合物、单宁类生物碱和皂苷类化合物。青柠叶提取物对伤寒沙门氏菌具有抑菌活性。抑菌活性试验表明,浓度为2.5%时,平均抑菌带为8 mm,浓度为5%时为9.6 mm,浓度为10%时为11.6 mm。
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审稿时长
24 weeks
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