A Micro Amperometric Immunosensor Based on Protein A/Gold nanoparticles/Self-assembled Monolayer-Modified Gold Electrode

2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems Pub Date : 2006-01-01 DOI:10.1109/NEMS.2006.334617

Yuanyuan Xu, S. Xia, C. Bian, Shaofeng Chen

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Abstract

A novel amperometric immunosensor fabricated by microelectromechanical systems (MEMS) technology with a novel modified procedure for immobilization of antibody on gold electrode has been developed. Based on MEMS technology, immunosensor with a three-microelectrode system integrated with two SU-8 micro pools was fabricated. Employing self-assembled monolayers technique, the working electrode was modified by 1,6-hexanedithiol to assemble gold nanoparticles layer, subsequently, a layer of protein A was immobilized on nanogold layer to further capture antibody. Compared with the amperometric immunosensors using traditionally metal rod/slice electrode or screen-printed electrode, it has attractive advantages, such as miniaturization, compatibility with CMOS techniques and easy to be designed into micro array. Moreover, the studied immunosensor showed high specificity, good reproducibility and broad linear range for the detection of human immunoglobulin (HIgG), which makes it potentially attractive for clinical immunoassays

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基于蛋白A/金纳米颗粒/自组装单层修饰金电极的微安培免疫传感器

利用微机电系统(MEMS)技术，改进了抗体在金电极上固定的方法，研制了一种新型的安培免疫传感器。基于MEMS技术，制作了基于两个SU-8微池集成的三微电极免疫传感器。采用自组装单层技术，用1,6-己二硫醇修饰工作电极，组装金纳米粒子层，随后在纳米金层上固定一层蛋白a，进一步捕获抗体。与传统的金属棒/片电极或丝网印刷电极的安培免疫传感器相比，它具有小型化、兼容CMOS技术和易于设计成微阵列等优点。此外，所研究的免疫传感器对人免疫球蛋白(HIgG)的检测具有高特异性、良好的重现性和宽的线性范围，在临床免疫分析中具有潜在的应用价值

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2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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