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2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems最新文献

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A RFID Tag Based Remote DNA sensing System 基于RFID标签的远程DNA传感系统
J. Chien, C. Tsai, Y.T. Cheng, C.R. Yang, P.Y. Wang, T.L. Chang, D.S. Lee, C. W. Chang, W.P. Chou, C. Chiang, Y.W. Lee, P. Chen
Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the microcapacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radiofrequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection
与传统的基于聚合酶链反应(PCR)和酶联免疫吸附法(ELISA)的生化检测方法昂贵且耗时不同,本研究采用纳米金(AuNP)免疫扩增法和RFID通信平台的DNA芯片检测技术,快速方便地检测DNA序列。首先将捕获的DNA (cDNA)固定在电容器所在的底物上。然后在3'端加入带有AuNP的靶DNA (tDNA)和探针DNA (pDNA),形成多层AuNP连接结构。由于微电容间隙之间的aunp连接结构改变了电容所在介质的介电常数,串联LC电路的谐振频率立即发生位移。本研究提出的片上实验室由射频识别(RFID)系统供电。LC谐振频率的位移决定了tDNA是否存在。因此,检测芯片不需要任何其他内部电源或昂贵的仪器进行电信号测量。本研究为生物化学检测提供了一个简单、快速、方便的平台
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引用次数: 3
Simultaneous Quantification for Hepatitis B Virus and Hepatitis C Virus Using Real-time PCR Lab-on-a-chip 实时荧光定量PCR检测乙肝病毒和丙肝病毒
J. Chien, D.S. Lee, W.P. Chou, P.Y. Wang, C.R. Yang, M.H. Wu, C. Tsai, T.L. Chang, Y.W. Lee, Y.T. Cheng, P. Chen
The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-chip system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1degC.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one
目前的实时PCR (polymerase chain reaction,聚合酶链反应)平台采用离散光学器件和不同荧光波长的检测器,可以同时检测和定量多个目标DNA。然而,由于不同染料的通道长度不同,光学损耗降低了荧光检测的性能。特别是在芯片实验室系统的PCR平台上,由于荧光在微流控通道中的分散,接收到的荧光远低于发射的荧光。因此,提高探测系统的接收强度是一个关键问题。该荧光检测系统由一个超灵敏光谱仪组成,可提供连续波长检测,可在一次反应中进行多个DNA定量和基因分型。对于基因分型能力的测试,B型HBV和C型HBV的熔化温度可以区分为1.1℃的差异。本研究的测试结果表明,与商业样品相比,5个检测样品的DNA定量灵敏度和可重复性相同
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引用次数: 3
A Hybrid Nano-Imprinting Lithography Based on Infrared Pulsed Laser Heating 基于红外脉冲激光加热的混合纳米压印光刻技术
Y. Lee, Chun-Hung Chen, Chuan-Pu Liu
In this paper we present a novel method of nano-imprinting which adopts important features of conventional nano-imprinting lithography (NIL) and the newly developed laser-assisted direct imprinting (LADI) method. It utilizes an Nd-YAG pulsed laser of wavelength 1064 nm which can easily penetrate and also heat up a silicon mold which is pressed against a resist layer deposited on a substrate. The fast rising temperature in the silicon mold can momentarily melt the resist layer so that the mold is imprinting into the resist layer. After the pattern is transformed, standard nano-imprinting lithography processes can be applied to the substrate for nano-fabrication. This new method has several advantages over existing nano-imprinting methods mostly due to the fast heating-up of silicon mold by high intensity IR laser pulse and therefore has no thermal drifting problem. Both the theoretical modeling and experimental results of this novel IR-laser assisted imprinting method will be presented
本文提出了一种新的纳米压印方法,它结合了传统纳米压印(NIL)的重要特点和新发展的激光辅助直接压印(LADI)方法。它利用波长1064nm的Nd-YAG脉冲激光器,可以很容易地穿透并加热压在衬底上的抗蚀剂层上的硅模。硅模内快速上升的温度可以瞬间熔化抗蚀剂层,从而使模具压印到抗蚀剂层中。图案转换后,可将标准的纳米压印工艺应用于基片上进行纳米加工。与现有的纳米压印方法相比,该方法具有许多优点,主要是由于高强度红外激光脉冲对硅模的快速加热,因此不存在热漂移问题。本文将介绍这种新型红外激光辅助印迹方法的理论建模和实验结果
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引用次数: 1
Self-Welded Metal-Catalyzed Carbon Nanotube Piezoresistors with Very Large Longitudinal Piezoresistivity of ~ 4×10-8 Pa-1 纵向压电阻~ 4×10-8 Pa-1的自焊接金属催化碳纳米管压电阻
M. Tabib-Azar, Run Wang, Yan Xie, L. You
Self-welded double-wall and multi-wall carbon nanotube (DWCNT and MWCNT) bridges were used for the first time as nano-scale piezoresistors to monitor vibration and deformation of silicon cantilever beams. The CNTs were grown using low-pressure metal-catalyzed chemical vapor deposition technique between silicon-on-insulator posts situated over cantilever beams such that when the beams were deformed, CNTs were axially strained. Telescoping of inner CNT cylinders occurring under these axial strains changed the CNTs resistance. The CNT-post weld strength, as measured using atomic force microscope, was larger than 100 nN/CNT and their full-scale resistance change was larger than 105 Ω. The effective longitudinal piezoresistivity of CNTs was larger than 4×10-8 Pa-1 which is more than 10 times larger than that of Π44 in silicon
首次将自焊双壁和多壁碳纳米管(DWCNT和MWCNT)桥用作纳米级压阻,用于监测硅悬臂梁的振动和变形。采用低压金属催化化学气相沉积技术在位于悬臂梁上的绝缘体上硅柱之间生长碳纳米管,这样当梁变形时,碳纳米管就会受到轴向应变。在这些轴向应变下发生的内碳纳米管圆柱体伸缩改变了碳纳米管的阻力。原子力显微镜测得碳纳米管焊缝强度大于100 nN/CNT,满量程电阻变化大于105 Ω。CNTs的有效纵向压阻率大于4×10-8 Pa-1,比Π44在硅中的有效纵向压阻率大10倍以上
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引用次数: 2
Dynamic Performance of Micro Coordinate Measurement Probe 微坐标测量探头的动态性能
P. Shih, W. Shih, Tzung-Han Lin, Han-Pang Huang
A three-dimensional microprobe, which is capable of measuring the forces exerting on the probing pin in all directions, is presented. The microprobe is fabricated on stainless steel and is covered by two sets of piezoelectric transducers. One of the transducers drives the probe, and the other senses the dynamic response to identify the contact between the pin and obstacles. A theoretical model is carried out to investigate the transition of the microprobe between contact and non-contact states. Accordingly, a bifurcation diagram is established to provide a design reference for the optimization of driving frequency. A finite element simulation is also carried out for the complete design optimization. Indeed, the fabricated microprobe can be used for the coordinate measurement of the high-aspect-ratio micro-structures
提出了一种三维微探头,可以测量各方向作用在探针上的力。微探头由不锈钢制成,并由两组压电换能器覆盖。其中一个传感器驱动探针,另一个传感器感知动态响应以识别引脚与障碍物之间的接触。建立了一个理论模型来研究微探针在接触和非接触状态之间的转变。据此,建立了分岔图,为驱动频率的优化提供了设计参考。对整个设计进行了有限元仿真。实际上,所制备的微探针可用于高纵横比微结构的坐标测量
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引用次数: 0
Study on Micro Electrochemical Machining at Micro to Meso-scale 微中观微细电化学加工研究
Wansheng Zhao, Xiaohai Li, Zhenlong Wang
Electrochemical machining (ECM) is an anodic dissolution process of metal as anode ion by ion, and micro-ECM is a promising micro machining method at micro to mesoscale. Thus, a micro-ECM setup was developed to fabricate micro parts and explore the feasibility of micro-ECM at micro to mesoscale, including the design of high-frequency micro-energy pulse power supply. By using the detection unit of machining state and optical encoder, a closed loop control system for micro-ECM was developed. Under low concentration of passivating electrolyte, low machining voltage and high-frequency short-pulse current, the machining gap can be reduced to about 10 mum. A deep micro-hole about 100 mum in diameter was drilled by edge-cut electrode on stainless steel with 750 mum thickness. The process of NC micro-EC milling is proposed, and microstructures with high-aspect ratio on stainless steel were fabricated by micro-EC milling, such as profiled micro-hole, micro spiral beam and micro array square columns
电化学加工(ECM)是一种以阳极离子为阳极的金属的阳极溶解过程,微电解加工是一种很有前途的微到中尺度的微加工方法。为此,开发了一种微型电解加工装置,用于制造微型零件,并探索了微型电解加工在微到中尺度上的可行性,包括高频微能量脉冲电源的设计。采用加工状态检测单元和光学编码器,研制了微细电解加工闭环控制系统。在低浓度钝化电解液、低加工电压和高频短脉冲电流的作用下,加工间隙可减小到10 μ m左右。在厚度为750 μ m的不锈钢上,用边切电极钻出直径约为100 μ m的深微孔。提出了数控微电火花铣削工艺,利用微电火花铣削技术在不锈钢表面加工出了高纵横比的异形微孔、微螺旋梁和微阵列方柱等微结构
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引用次数: 15
Photonic Properties of Silicon-on-Glass Nano-Films and its Fabrication 玻璃上硅纳米薄膜的光子特性及其制备
Simiao Xiao, Guangbo Li, Fan Wang, Xiaoqing Jiang, Ming-hua Wang, Jianyi Yang
The photonic properties of the silicon-on-glass (SOG) nano-films are analyzed first and the reason why SOG nano-films are required for the nanophotonics is pointed. Then the method to fabricate silicon-on-glass nano-film materials for the applications of nano photonics is established. Anodic boning and selective etching are employed. SOG-based nano-films are then studied.
首先分析了玻璃上硅(SOG)纳米薄膜的光子特性,指出了纳米光子学需要SOG纳米薄膜的原因。建立了用于纳米光子学应用的玻璃上硅纳米薄膜材料的制备方法。采用阳极焊接和选择性蚀刻。然后对sog基纳米薄膜进行了研究。
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引用次数: 1
A Micro Amperometric Immunosensor Based on Protein A/Gold nanoparticles/Self-assembled Monolayer-Modified Gold Electrode 基于蛋白A/金纳米颗粒/自组装单层修饰金电极的微安培免疫传感器
Yuanyuan Xu, S. Xia, C. Bian, Shaofeng Chen
A novel amperometric immunosensor fabricated by microelectromechanical systems (MEMS) technology with a novel modified procedure for immobilization of antibody on gold electrode has been developed. Based on MEMS technology, immunosensor with a three-microelectrode system integrated with two SU-8 micro pools was fabricated. Employing self-assembled monolayers technique, the working electrode was modified by 1,6-hexanedithiol to assemble gold nanoparticles layer, subsequently, a layer of protein A was immobilized on nanogold layer to further capture antibody. Compared with the amperometric immunosensors using traditionally metal rod/slice electrode or screen-printed electrode, it has attractive advantages, such as miniaturization, compatibility with CMOS techniques and easy to be designed into micro array. Moreover, the studied immunosensor showed high specificity, good reproducibility and broad linear range for the detection of human immunoglobulin (HIgG), which makes it potentially attractive for clinical immunoassays
利用微机电系统(MEMS)技术,改进了抗体在金电极上固定的方法,研制了一种新型的安培免疫传感器。基于MEMS技术,制作了基于两个SU-8微池集成的三微电极免疫传感器。采用自组装单层技术,用1,6-己二硫醇修饰工作电极,组装金纳米粒子层,随后在纳米金层上固定一层蛋白a,进一步捕获抗体。与传统的金属棒/片电极或丝网印刷电极的安培免疫传感器相比,它具有小型化、兼容CMOS技术和易于设计成微阵列等优点。此外,所研究的免疫传感器对人免疫球蛋白(HIgG)的检测具有高特异性、良好的重现性和宽的线性范围,在临床免疫分析中具有潜在的应用价值
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引用次数: 0
Research on Application of MEMS Accelerometer in Target Classification by Advanced Information Processing MEMS加速度计在先进信息处理目标分类中的应用研究
J. Lan, Zhaohui Zhang, Tian Lan
This paper presents a novel application of MEMS accelerometer in target classification by means of advanced information processing. The detection system based on MEMS accelerometer is small in size, light in weight, has low power consumption and low cost, and can work under severe circumstances for many different applications. In order to extract features of seismic signals stimulated by different vehicle targets and to recognize targets, seismic properties of typical vehicle targets are researched in the paper. A technique of artificial neural networks combined with genetic algorithm (ANNCGA) is applied to classification of seismic signals that belong to different kinds of vehicle targets. The technique and its architecture have been presented. The algorithm had been used for classification of seismic signals of vehicle targets in the outdoor environment. Through experiments, it can be proven that seismic properties of target acquired are correct, ANNCGA method is effective to solve the problem of target classification, and MEMS accelerometer can be used in vehicle target classification
本文介绍了MEMS加速度计利用先进的信息处理技术在目标分类中的新应用。基于MEMS加速度计的检测系统体积小,重量轻,功耗低,成本低,可以在许多不同的应用环境下工作。为了提取不同车辆目标激发的地震信号特征,实现目标识别,本文对典型车辆目标的地震特性进行了研究。将人工神经网络与遗传算法(ANNCGA)相结合的方法应用于不同类型车辆目标的地震信号分类。介绍了该技术及其体系结构。该算法已用于室外环境下车辆目标的地震信号分类。通过实验,可以证明所获取目标的地震特性是正确的,ANNCGA方法可以有效地解决目标分类问题,MEMS加速度计可以用于车辆目标分类
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引用次数: 5
Study on the Nano-topography of the Electrode Surface and the Breakdown Voltage in RF MEMS Switches 射频MEMS开关电极表面纳米形貌及击穿电压的研究
Zhihao Hou, Zewen Liu, Guangwei Hu, Litian Liu, Zhijian Li
Study on the relationship between the breakdown voltage and the nano-topography of electrode surface is presented. The bottom electrode is electroplated with three different current densities. The measured RMS roughness for the obtained electrodes is 27.4nm, 16.0nm and 5.4nm correspondingly. PECVD 300degC Si3N4 is deposited onto the electrodes as dielectric layer. After measuring the surface nano-topography of both the metallic surface and the dielectric surface, it is founded that the RMS roughness of Si3N4 layer is determined by that of the bottom electrode. Applying a ramping DC voltage between the up- and bottom- electrode, different breakdown voltages are obtained. For the switches with electrode roughness of 27.4nm, 16.0nm and 5.4nm, the breakdown event maximum is in the voltage ranges of 20-40V, 30-60V and 80-110V respectively
研究了击穿电压与电极表面纳米形貌的关系。底部电极电镀有三种不同的电流密度。所得电极的RMS粗糙度分别为27.4nm、16.0nm和5.4nm。将PECVD 300℃Si3N4作为介电层沉积在电极上。通过测量金属表面和介质表面的纳米形貌,发现Si3N4层的RMS粗糙度是由底部电极的RMS粗糙度决定的。在上电极和下电极之间施加直流电压,可以得到不同的击穿电压。对于电极粗糙度为27.4nm、16.0nm和5.4nm的开关,击穿事件最大值分别发生在20-40V、30-60V和80-110V电压范围内
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引用次数: 2
期刊
2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems
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