QTL Mining and Validation of Grain Nutritional Quality Characters in Rice (Oryza sativa L.) Using Two Introgression Line Populations

IF 3.6 2区农林科学 Q1 AGRONOMY Agriculture-Basel Pub Date : 2023-08-31 DOI:10.3390/agriculture13091725

Mufid Alam, Xuan Tan, Hao Zhang, Guangming Lou, Hanyuan Yang, Yin Zhou, A. Hussain, Parashuram Bhantana, G. Jiang, Yuqing He

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引用次数: 1

Abstract

Nutritional grain quality is mainly influenced by the protein fraction content and grain protein content. Quantitative trait loci (QTL) mining for five traits, about 245 and 284 BC3F3 individual families of two introgression line (IL) populations were derived from Kongyu 131/Cypress (population-I) and Kongyu 131/Vary Tarva Osla (population-II), respectively. A genetic linkage map was developed using 127 simple sequence repeat (SSR) markers in population-I and 119 SSR markers in population-II. In total, 20 and 5 QTLs were detected in population-I and population-II, respectively. About twenty QTLs were mapped in population-I: five QTLs for albumin, seven QTLs for globulin, six QTLs for prolamin, one QTL for glutelin, and one QTL for grain protein content. In total, five QTLs were mapped in population-II: one QTL for albumin and four QTLs for grain protein content. Out of 25 QTLs, 19 QTLs exhibit co-localization with the previously reported QTLs. QTL-like qGPC7.3 was delineated for total protein content. This QTL was derived from population-I and was successfully validated in NILs (near-isogenic lines). The grain protein phenotype showed a significant variation between two NILs. This investigation serves as groundwork for additional cloning of nutritional quality-related genes in rice grains.

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水稻籽粒营养品质性状的QTL挖掘与验证利用两个渐渗系种群

营养品质主要受蛋白质组分含量和籽粒蛋白质含量的影响。从孔玉131/柏树(居群- i)和孔玉131/白杨(居群- ii) 2个种群的5个性状，分别获得245和284个BC3F3个体家族的数量性状位点(QTL)挖掘。利用群体i中的127个SSR标记和群体ii中的119个SSR标记，构建了遗传连锁图谱。在种群i和种群ii中分别检测到20个和5个qtl。在群体i中共定位了20个QTL，其中白蛋白QTL 5个，球蛋白QTL 7个，蛋白QTL 6个，谷蛋白QTL 1个，籽粒蛋白质含量QTL 1个。在群体ii中共定位了5个QTL: 1个白蛋白QTL和4个籽粒蛋白质含量QTL。在25个qtl中，19个qtl与先前报道的qtl共定位。总蛋白含量为qtl样qGPC7.3。该QTL来源于种群i，并在近等基因系中得到验证。籽粒蛋白表型在两个NILs之间存在显著差异。本研究为进一步克隆水稻营养品质相关基因奠定了基础。

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来源期刊

Agriculture-Basel Agricultural and Biological Sciences-Food Science

CiteScore

4.90

自引率

13.90%

发文量

1793

审稿时长

11 weeks

期刊介绍： Agriculture (ISSN 2077-0472) is an international and cross-disciplinary scholarly and scientific open access journal on the science of cultivating the soil, growing, harvesting crops, and raising livestock. We will aim to look at production, processing, marketing and use of foods, fibers, plants and animals. The journal Agriculturewill publish reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.