Passiflora quadrangularis L. prevents experimental hypertension and vascular remodelling in rats exposed to nitric oxide deficit

Lesly L. Bareño, P. Puebla, Carlos M. Guerra, A. S. Feliciano, G. Isaza, M. Guerrero
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引用次数: 4

Abstract

Background: Passiflora quadrangularis L. is among the species used in Colombian folk medicine for hypertension, but until now it has not been studied in experimental models. Objectives: To assess the capacity of P. quadrangularis L. EtOH extract to prevent the hypertension and vascular remodelling induced by nitric oxide (NO) deficit in Wistar rats. Methods: The nitric oxide (NO) synthase inhibitor L-NAME (10 mg/kg, i.p (intraperitoneal), every 48h) was administered for seven weeks to the following groups of rats: P. quadrangularis L.75, 150 and 300 mg/kg/d, p.o. (oral route); enalapril as reference agent, 10 mg/kg/d, p.o. and vehicle as control (mixture of propylene glycol 10%, glycerine 10% and polysorbate 2%). Arterial blood pressure (BP) and heart rate (HR) were measured twice a week. After sacrifice, the aortic rings were isolated, contraction was triggered with phenylephrine (PE 10-6 M) and then the relaxant response achieved with cumulative concentrations of acetylcholine (ACh, 10-10 – 10-5 M) or sodium nitroprusside (SNP, 10-10 – 10-5 M) was assessed. Histopathologic measures of thickness/lumen ratio from both the left ventricle and aorta walls, as well as phytochemical screening, were also performed. Results: As for enalapril, all doses of P. quadrangularis L. prevented the hypertension induced by L-NAME (122±1.2 versus 155±1.3 mmHg at seventh week). P. quadrangularis L. significantly increased the relaxant effect induced by ACh in isolated aorta and decreased the thickness/lumen ratio of aorta wall specimens. Conclusions: P. quadrangularis L. prevents experimental hypertension induced in rats with nitric oxide deficits improving the endothelium vasodilatation response and protecting against vascular remodelling.
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西番莲可预防一氧化氮缺陷大鼠的实验性高血压和血管重构
背景:西番莲(Passiflora quadrangularis L.)是哥伦比亚民间治疗高血压药物中使用的植物之一,但到目前为止还没有对其进行实验模型研究。目的:探讨四角藤提取物对Wistar大鼠一氧化氮(NO)缺陷所致高血压和血管重构的预防作用。方法:将一氧化氮(NO)合成酶抑制剂L-NAME (10 mg/kg,腹腔灌胃,每48h 1次)连续7周给药:四角鼠L.75、150、300 mg/kg/d,口服;依那普利为对照剂,10 mg/kg/d, p.o.和对照剂(丙二醇10%、甘油10%、聚山梨酯2%的混合物)。每周一次测量动脉血压(BP)和心率(HR)。牺牲后,分离主动脉环,用苯肾上腺素(PE 10-6 M)触发收缩,然后用累积浓度的乙酰胆碱(ACh, 10-10 -5 M)或硝普钠(SNP, 10-10 - 10-5 M)评估松弛反应。左心室和主动脉壁厚度/管腔比的组织病理学测量,以及植物化学筛选,也进行了。结果:对于依那普利,所有剂量的四角草均可预防L-NAME诱导的高血压(第7周时为122±1.2 mmHg vs 155±1.3 mmHg)。四棱草显著增强乙酰胆碱对离体主动脉的松弛作用,降低主动脉壁标本的厚度/管腔比。结论:四角草可预防一氧化氮缺失大鼠实验性高血压,改善内皮血管舒张反应,防止血管重构。
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