Antiangiogenic and Apoptotic Effects of Oleuropein on Breast Cancer Cells

Abstract

Oleuropein is a fundamental polyphenol contained in olive fruit, oil, and leaf, with a bitter taste. A decrease in its level is observed as the fruit ripens. Leaves of oil tree were used in this study as a possible origin of oleuropein. In this study, cytotoxic, genotoxic, apoptotic and antiangiogenic impacts of isolated oleuropein (0.5xIC50, IC50 and 2xIC50) on MCF-7 human breast adenocarcinoma cells were examined by means of 3-[4,5Dimethylthiazol-2-yl] 1-2,5diphenyltetrazolium bromide (XTT) kit, Comet assay, Poly (ADP-Ribose) Polymerase (PARP) cleavage ELISA Kit and Vascular Endothelial Growth Factor (VEGF) Human ELISA Kit for 24, 48 and 72 h, respectively. The IC50 value of oleuropein on MCF-7 cells was found to be 11.02±0.52, 9.43±0.41 and 6.81±0.18 μg/mL for 24, 48 and 72 h, respectively. The time and concentrationrelated cytotoxic impacts of oleuropein were determined to be significant. Cleaved PARP level was Original Research Article Liman et al.; BJPR, 16(4): 1-10, 2017; Article no.BJPR.33403 2 significantly increased especially with an increase in the concentration of oleuropein. However, a significant decrease in VEGF level was observed (p<0.05). Furthermore, oleuropein increased damage on DNA for 48 and 72 h. Findings showed oleuropein as a potential antiangiogenic, apoptotic and genotoxic agent for the breast cancer cells.