Isolation and Identification of 9-methoxycanthin-6-0ne Compound From Eurycoma Longifolia Roots

Abstract

9-Methoxycanthin-6-one compound has been isolated from Pasak Bumi (E. Longifolia) roots. The isolation process using maseration, colum chromatography vacuum, and recrystallization techniques. E. Longifolia root maserated with n-hexane and methanol respectively. The methanol extract fractionated using column chromatography vacuum with gradient elution (hexane-etil acetate) to obtain 9 fractions. Fraction 5 further purification using recrystallization technique to obtain yellow crystal, identified by LC-MS and NMR spectroscopy as 9Methoxycanthin-6-one Keyword: E. Longifolia, isolation, spectroscopy, 9Methoxycan thin-6-one.