{"title":"Polypeptide composition of major oilseed proteins and functional properties of extracted protein products: A concise review","authors":"Rotimi E. Aluko","doi":"10.1002/aocs.12740","DOIUrl":null,"url":null,"abstract":"<p>Oilseeds are grown mainly for their oil content but the residues (meals) that remain after defatting are excellent sources of plant protein ingredients. However, to serve as useful ingredients, the extracted proteins must meet industry expectations in terms of functional performance. Protein functionality is influenced by structural conformation, amino acid composition, type of polypeptides, presence of non-protein materials (carbohydrates, lipids, and polyphenols), which in turn can be modified by the extraction method. Defatted oilseed meals are extracted mostly through the pH shift method, which involves alkaline solubilization followed by acid-induced protein precipitation at the isoelectric point. A less popular method is called the protein micellar mass whereby the oilseed meal proteins are extracted with a NaCl solution, which is later diluted to reduce the ionic strength to a level where the proteins are no longer soluble and hence precipitate. A third method utilizes carbohydrases and phytases to first digest non-protein materials from the oilseed meal into smaller units that are then removed by membrane ultrafiltration to leave behind a protein-rich extract. These methods produce mainly two types of isolated oilseed proteins, concentrates (60%–89% protein content) and isolates (≥90% protein content), which can differ in terms of their protein conformation, solubility, and functionality as food ingredients. Therefore, this review provides an overview of the extraction and isolation as well as structural and functional properties of soybean, peanut, canola, hemp seed, sunflower, and sesame seed proteins.</p>","PeriodicalId":17182,"journal":{"name":"Journal of the American Oil Chemists Society","volume":"101 1","pages":"23-39"},"PeriodicalIF":1.9000,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Oil Chemists Society","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/aocs.12740","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
Oilseeds are grown mainly for their oil content but the residues (meals) that remain after defatting are excellent sources of plant protein ingredients. However, to serve as useful ingredients, the extracted proteins must meet industry expectations in terms of functional performance. Protein functionality is influenced by structural conformation, amino acid composition, type of polypeptides, presence of non-protein materials (carbohydrates, lipids, and polyphenols), which in turn can be modified by the extraction method. Defatted oilseed meals are extracted mostly through the pH shift method, which involves alkaline solubilization followed by acid-induced protein precipitation at the isoelectric point. A less popular method is called the protein micellar mass whereby the oilseed meal proteins are extracted with a NaCl solution, which is later diluted to reduce the ionic strength to a level where the proteins are no longer soluble and hence precipitate. A third method utilizes carbohydrases and phytases to first digest non-protein materials from the oilseed meal into smaller units that are then removed by membrane ultrafiltration to leave behind a protein-rich extract. These methods produce mainly two types of isolated oilseed proteins, concentrates (60%–89% protein content) and isolates (≥90% protein content), which can differ in terms of their protein conformation, solubility, and functionality as food ingredients. Therefore, this review provides an overview of the extraction and isolation as well as structural and functional properties of soybean, peanut, canola, hemp seed, sunflower, and sesame seed proteins.
期刊介绍:
The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate.
JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.